1u0d: Difference between revisions

Revision as of 10:35, 3 May 2008

Y33H Mutatant of Homing endonuclease I-CreI

OverviewOverview

Homing endonucleases are highly specific DNA endonucleases, encoded within mobile introns or inteins, that induce targeted recombination, double-strand repair and gene conversion of their cognate target sites. Due to their biological function and high level of target specificity, these enzymes are under intense investigation as tools for gene targeting. These studies require that naturally occurring enzymes be redesigned to recognize novel target sites. Here, we report studies in which the homodimeric LAGLIDADG homing endonuclease I-CreI is altered at individual side-chains corresponding to contact points to distinct base-pairs in its target site. The resulting enzyme constructs drive specific elimination of selected DNA targets in vivo and display shifted specificities of DNA binding and cleavage in vitro. Crystal structures of two of these constructs demonstrate that substitution of individual side-chain/DNA contact patterns can occur with almost no structural deformation or rearrangement of the surrounding complex, facilitating an isolated, modular redesign strategy for homing endonuclease activity and specificity.

About this StructureAbout this Structure

1U0D is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

ReferenceReference

Isolation and characterization of new homing endonuclease specificities at individual target site positions., Sussman D, Chadsey M, Fauce S, Engel A, Bruett A, Monnat R Jr, Stoddard BL, Seligman LM, J Mol Biol. 2004 Sep 3;342(1):31-41. PMID:15313605 Page seeded by OCA on Sat May 3 10:35:40 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1u0d.gif|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_1u0d", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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+or leave the SCENE parameter empty for the default display.
-|GENE=
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-|DOMAIN=
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-|RELATEDENTRY=[[1u0c|1U0C]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0d OCA], [http://www.ebi.ac.uk/pdbsum/1u0d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u0d RCSB]</span>
-}}
 '''Y33H Mutatant of Homing endonuclease I-CreI'''
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 [[Category: Stoddard, B L.]]
 [[Category: Sussman, D.]]
-[[Category: dna endonuclease i-crei]]
+[[Category: Dna endonuclease i-crei]]
-[[Category: protein/dna]]
+[[Category: Protein/dna]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:35:40 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:03:37 2008''