Sandbox Reserved 1063: Difference between revisions
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=== Helix-Turn-Helix Domain === | === Helix-Turn-Helix Domain === | ||
The AdcR MarR transcriptional regulator's structure resembles the other proteins in the same family as mentioned before; however, the most notable differences are found in the winged helix-turn-helix (wHTH) motif that assists in binding DNA. Although AdcR is a highly alpha helical protein, the "wings" of the DNA binding domain consist of two anti parallel beta strands that are made up of several positively charged residues such as Arg. The major groove of DNA is bound to the recognition helix while the wings grip onto the minor grooves. The charge map on the right highlights the <font color='blue'>positively</font> charged areas, which stabilize the negatively charged backbone of the major and minor grooves of DNA. There is one on each domain of the protein. | The AdcR MarR transcriptional regulator's structure resembles the other proteins in the same family as mentioned before; however, the most notable differences are found in the winged helix-turn-helix (wHTH) motif that assists in binding DNA. Although AdcR is a highly alpha helical protein, the "wings" of the DNA binding domain consist of two anti parallel beta strands that are made up of several positively charged residues such as Arg. The major groove of DNA is bound to the recognition helix while the wings grip onto the minor grooves. The charge map on the right highlights the <font color='blue'>positively</font> charged areas, which stabilize the negatively charged backbone of the major and minor grooves of DNA. There is one on each domain of the protein. | ||
The <scene name='69/694230/Whth/1'>wHTH</scene> domain is made up of the alpha 2 and alpha 4 helices along with anti-parallel beta sheets on each side. These structures can been seen in the java applet as all green structures in the rainbow scheme for clarity purposes. Only one monomer is shown. The recognition helix, or the alpha 4 helix, binds the major groove of DNA through hydrogen bonding and Van der Waals interactions between exposed bases. The wings of the helix bind the minor groove of DNA while the other helices stabilize the DNA and Protein upon binding. The two anti parallel beta sheets contain several Arginine residues | The <scene name='69/694230/Whth/1'>wHTH</scene> domain is made up of the alpha 2 and alpha 4 helices along with anti-parallel beta sheets on each side. These structures can been seen in the java applet as all green structures in the rainbow scheme for clarity purposes. Only one monomer is shown. The recognition helix, or the alpha 4 helix, binds the major groove of DNA through hydrogen bonding and Van der Waals interactions between exposed bases. The wings of the helix bind the minor groove of DNA while the other helices stabilize the DNA and Protein upon binding. The two anti parallel beta sheets contain several <scene name='69/694230/Positive_residues_on_wing/1'>Arginine, Asparagine, and Lysine residues</scene> that stabilize this interaction between DNA. | ||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||