Sandbox Reserved 1063: Difference between revisions

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OCA, Zach LaRoche, Paxton Schowe, Geoffrey C. Hoops, Alexi Zaniker, Shandeep Singh, Isaac C. Gluesenkamp

Revision as of 20:41, 31 March 2017 view source Isaac C. Gluesenkamp (talk \| contribs) 69 edits No edit summary ← Older edit		Revision as of 20:41, 31 March 2017 view source Alexi Zaniker (talk \| contribs) 94 edits No edit summary Newer edit →
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	[[Image:H Bonding of DNA.png\|300 px\|left\|thumb\|The Hydrogen Bonding Network is shown with dotted green lines approximately 2.8 angstroms between residues. The network consists of 4 major residues as follows from right to left: E24, N38, Q40, S74. ]]		[[Image:H Bonding of DNA.png\|300 px\|left\|thumb\|The Hydrogen Bonding Network is shown with dotted green lines approximately 2.8 angstroms between residues. The network consists of 4 major residues as follows from right to left: E24, N38, Q40, S74. ]]

	The binding of Zinc allows for the conformational change that induces the binding of DNA in order to activate genes. The binding of Zinc metals creates a hydrogen bond network within the protein that connects the metal binding sites and the [https://en.wikipedia.org/wiki/DNA-binding_domain DNA binding domain]. Most importantly, the hydrogen bonding network connects the metal binding pockets to the alpha 4 helix. Alpha 4 helix plays a crucial role in binding DNA because it acts as the recognition helix. The specific sequence of DNA that is recognized by alpha helix 4 is unknown at the moment; however, scientists believe that the hydrogen bond network acts as an allosteric activator for the protein to bind DNA. The hydrogen bond network connects the alpha 2 and alpha 4 helix via hydrogen bonding between specific residues. After zinc is bound, a glutamate (<font color='blue'>E24</font>) residue from a random coil accepts a hydrogen bond from the carboxamide end of an asparagine (<font color='green'>N38</font>) residue from the alpha 2 helix. Then, a glutamine (<font color='~~yellow~~'>Q40</font>) residue from alpha 2 helix accepts a hydrogen bond from a serine (<font color='red'>S74</font>) residue from the alpha 4 helix. The color coding in the previous sentence represents the <scene name='69/694230/Hydrogen_bonding_1/1'>~~Hyrdogen~~ Bonding Network</scene>, which is seen across the MarR family as a whole. Now the protein is ready to bind DNA.		The binding of Zinc allows for the conformational change that induces the binding of DNA in order to activate genes. The binding of Zinc metals creates a hydrogen bond network within the protein that connects the metal binding sites and the [https://en.wikipedia.org/wiki/DNA-binding_domain DNA binding domain]. Most importantly, the hydrogen bonding network connects the metal binding pockets to the alpha 4 helix. Alpha 4 helix plays a crucial role in binding DNA because it acts as the recognition helix. The specific sequence of DNA that is recognized by alpha helix 4 is unknown at the moment; however, scientists believe that the hydrogen bond network acts as an allosteric activator for the protein to bind DNA. The hydrogen bond network connects the alpha 2 and alpha 4 helix via hydrogen bonding between specific residues. After zinc is bound, a glutamate (<font color='blue'>E24</font>) residue from a random coil accepts a hydrogen bond from the carboxamide end of an asparagine (<font color='green'>N38</font>) residue from the alpha 2 helix. Then, a glutamine (<font color='gold'>Q40</font>) residue from alpha 2 helix accepts a hydrogen bond from a serine (<font color='red'>S74</font>) residue from the alpha 4 helix. The color coding in the previous sentence represents the <scene name='69/694230/Hydrogen_bonding_1/1'>Hydrogen Bonding Network</scene>, which is seen across the MarR family as a whole. Now the protein is ready to bind DNA.