1ts6: Difference between revisions

Revision as of 10:18, 3 May 2008

Structure of the pB2 intermediate from time-resolved Laue crystallography

OverviewOverview

Determining 3D intermediate structures during the biological action of proteins in real time under ambient conditions is essential for understanding how proteins function. Here we use time-resolved Laue crystallography to extract short-lived intermediate structures and thereby unveil signal transduction in the blue light photoreceptor photoactive yellow protein (PYP) from Halorhodospira halophila. By analyzing a comprehensive set of Laue data during the PYP photocycle (forty-seven time points from one nanosecond to one second), we track all atoms in PYP during its photocycle and directly observe how absorption of a blue light photon by its p-coumaric acid chromophore triggers a reversible photocycle. We identify a complex chemical mechanism characterized by five distinct structural intermediates. Structural changes at the chromophore in the early, red-shifted intermediates are transduced to the exterior of the protein in the late, blue-shifted intermediates through an initial "volume-conserving" isomerization of the chromophore and the progressive disruption of hydrogen bonds between the chromophore and its surrounding binding pocket. These results yield a comprehensive view of the PYP photocycle when seen in the light of previous biophysical studies on the system.

About this StructureAbout this Structure

1TS6 is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.

ReferenceReference

Visualizing reaction pathways in photoactive yellow protein from nanoseconds to seconds., Ihee H, Rajagopal S, Srajer V, Pahl R, Anderson S, Schmidt M, Schotte F, Anfinrud PA, Wulff M, Moffat K, Proc Natl Acad Sci U S A. 2005 May 17;102(20):7145-50. Epub 2005 May 3. PMID:15870207 Page seeded by OCA on Sat May 3 10:18:25 2008

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OCA

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 [[Image:1ts6.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ts6 |SIZE=350|CAPTION= <scene name='initialview01'>1ts6</scene>, resolution 1.6&Aring;
+The line below this paragraph, containing "STRUCTURE_1ts6", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=HC4:4&#39;-HYDROXYCINNAMIC+ACID'>HC4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= PYP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 Halorhodospira halophila])
+-->
-|DOMAIN=
+{{STRUCTURE_1ts6|  PDB=1ts6  |  SCENE=  }}
-|RELATEDENTRY=[[1ts0|1TS0]], [[1ts7|1TS7]], [[1ts8|1TS8]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ts6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ts6 OCA], [http://www.ebi.ac.uk/pdbsum/1ts6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ts6 RCSB]</span>
-}}
 '''Structure of the pB2 intermediate from time-resolved Laue crystallography'''
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 [[Category: Srajer, V.]]
 [[Category: Wulff, M.]]
-[[Category: photoreceptor]]
+[[Category: Photoreceptor]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:18:25 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:00:20 2008''