Sandbox Reserved 1052: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA, Morgan Blake, Sarah Zimmerman, Geoffrey C. Hoops, Mary Liggett, Jakob Jozwiakowski

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 <Structure load='2KJB' size='350' frame='true' align='left' caption='3D Representation of CzrA with Zn Bound' scene='Insert optional scene name here' />
 ==CzrA==
-This is a default text for your page ''''''. Click above on '''edit this page''' to modify. Be careful with the &lt; and &gt; signs.
-You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.
 == Biological Function ==
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 == DNA Bound State ==
-The <scene name='69/694219/Serandhisresidues/2'>main DNA interactions</scene> have been found to occur at the SER 54 and 57 along with HIS 58. These residues are found in the N terminal of the R helix. The residues involved in the <scene name='69/694219/Dna_binding_pocket/1'>DNA binding pocket</scene> are VAL 42 and GLN 53.
+The <scene name='69/694219/Serandhisresidues/2'>main DNA interactions</scene> have been found to occur at the Ser 54 and 57 along with His 58. These residues are likely to interact with the 5'-TGAA sequence found in the half-site of the DNA. These residues are found in the N terminal of the R helix. The residues involved in the <scene name='69/694219/Dna_binding_pocket/1'>DNA binding pocket</scene> are Val 42 and Gln 53. This was experimentally determined by replacing the Gln and Val with Ala residues and measuring the binding capacity; the Ka decresed by 11 and 160-fold respectively<ref name="LoC">[Arunkumar A., Campanello G., Giedroc D. (2009). Solution Structure of a
+paradigm ArsR family zinc sensor in the DNA-bound state. PNAS 106:43
+-18182.]</ref> The previously mentioned Ser and His residues were found to bind the DNA with a similar affinity as the fully inhibited Zn<sup>2+</sup>.
 == Zinc Ligand(s) ==
 == Other Ligands ==
-This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
 </StructureSection>
 == References ==
-<references/>
+Arunkumar A., Campanello G., Giedroc D. (2009). Solution Structure of a
+paradigm ArsR family zinc sensor in the DNA-bound state. PNAS 106:43
+-18182