4z4u: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of GII.10 P domain in complex with 37.5mM fucose== | ==Crystal structure of GII.10 P domain in complex with 37.5mM fucose== | ||
<StructureSection load='4z4u' size='340' side='right' caption='[[4z4u]], [[Resolution|resolution]] 1.89Å' scene=''> | <StructureSection load='4z4u' size='340' side='right'caption='[[4z4u]], [[Resolution|resolution]] 1.89Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4z4u]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z4U FirstGlance]. <br> | <table><tr><td colspan='2'>[[4z4u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Norovirus_gii.10 Norovirus gii.10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z4U FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z4u OCA], [http://pdbe.org/4z4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z4u RCSB], [http://www.ebi.ac.uk/pdbsum/4z4u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z4u ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z4u OCA], [http://pdbe.org/4z4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z4u RCSB], [http://www.ebi.ac.uk/pdbsum/4z4u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z4u ProSAT]</span></td></tr> | ||
| Line 20: | Line 20: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Norovirus gii 10]] | |||
[[Category: Hansman, G S]] | [[Category: Hansman, G S]] | ||
[[Category: Koromyslova, A D]] | [[Category: Koromyslova, A D]] | ||
Revision as of 10:06, 19 June 2019
Crystal structure of GII.10 P domain in complex with 37.5mM fucoseCrystal structure of GII.10 P domain in complex with 37.5mM fucose
Structural highlights
Publication Abstract from PubMedHuman noroviruses bind histo-blood group antigens (HBGAs) and this interaction is thought to be important for an infection. We identified two additional fucose-binding pockets (termed fucose-3/4 sites) on a genogroup II human (GII.10) norovirus-protruding (P) dimer using X-ray crystallography. Fucose-3/4 sites were located between two previously determined HBGA binding pockets (termed fucose-1/2 sites). We found that four fucose molecules were capable of binding altogether at fucose-1/2/3/4 sites on the P dimer, though the fucose molecules bound in a dose-dependent and step-wise manner. We also showed that HBGA B-trisaccharide molecules bound in a similar way at the fucose-1/2 sites. Interestingly, we discovered that the monomers of the P dimer were asymmetrical in an unliganded state and when a single B-trisaccharide molecule bound, but were symmetrical when two B-trisaccharide molecules bound. We postulate that the symmetrical dimers might favor HBGA binding interactions at fucose-1/2 sites. The sweet quartet: Binding of fucose to the norovirus capsid.,Koromyslova AD, Leuthold MM, Bowler MW, Hansman GS Virology. 2015 May 13;483:203-208. doi: 10.1016/j.virol.2015.04.006. PMID:25980740[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||