1t0r: Difference between revisions

Revision as of 09:21, 3 May 2008

Crystal Structure of the Toluene/o-xylene Monooxygenase Hydroxuylase from Pseudomonas stutzeri-azide bound

OverviewOverview

The four-component toluene/o-xylene monooxygenase (ToMO) from Pseudomonas stutzeri OX1 is capable of oxidizing arenes, alkenes, and haloalkanes at a carboxylate-bridged diiron center similar to that of soluble methane monooxygenase (sMMO). The remarkable variety of substrates accommodated by ToMO invites applications ranging from bioremediation to the regio- and enantiospecific oxidation of hydrocarbons on an industrial scale. We report here the crystal structures of the ToMO hydroxylase (ToMOH), azido ToMOH, and ToMOH containing the product analogue 4-bromophenol to 2.3 A or greater resolution. The catalytic diiron(III) core resembles that of the sMMO hydroxylase, but aspects of the alpha2beta2gamma2 tertiary structure are notably different. Of particular interest is a 6-10 A-wide channel of approximately 35 A in length extending from the active site to the protein surface. The presence of three bromophenol molecules in this space confirms this route as a pathway for substrate entrance and product egress. An analysis of the ToMOH active site cavity offers insights into the different substrate specificities of multicomponent monooxygenases and explains the behavior of mutant forms of homologous enzymes described in the literature.

About this StructureAbout this Structure

1T0R is a Protein complex structure of sequences from Pseudomonas stutzeri. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the toluene/o-xylene monooxygenase hydroxylase from Pseudomonas stutzeri OX1. Insight into the substrate specificity, substrate channeling, and active site tuning of multicomponent monooxygenases., Sazinsky MH, Bard J, Di Donato A, Lippard SJ, J Biol Chem. 2004 Jul 16;279(29):30600-10. Epub 2004 Apr 19. PMID:15096510 Page seeded by OCA on Sat May 3 09:21:49 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1t0r.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1t0r |SIZE=350|CAPTION= <scene name='initialview01'>1t0r</scene>, resolution 2.30&Aring;
+The line below this paragraph, containing "STRUCTURE_1t0r", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= touA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), touE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), touB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri])
+-->
-|DOMAIN=
+{{STRUCTURE_1t0r|  PDB=1t0r  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0r OCA], [http://www.ebi.ac.uk/pdbsum/1t0r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t0r RCSB]</span>
-}}
 '''Crystal Structure of the Toluene/o-xylene Monooxygenase Hydroxuylase from Pseudomonas stutzeri-azide bound'''
@@ Line 30: / Line 27: @@
 [[Category: Sazinsky, M H.]]
 [[Category: 4-helix bundle]]
-[[Category: azide]]
+[[Category: Azide]]
-[[Category: carboxylate bridge]]
+[[Category: Carboxylate bridge]]
-[[Category: diiron]]
+[[Category: Diiron]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 09:21:49 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:49:33 2008''