1svy: Difference between revisions
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'''SEVERIN DOMAIN 2, 1.75 ANGSTROM CRYSTAL STRUCTURE''' | '''SEVERIN DOMAIN 2, 1.75 ANGSTROM CRYSTAL STRUCTURE''' | ||
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[[Category: Schleicher, M.]] | [[Category: Schleicher, M.]] | ||
[[Category: Sullivan, M.]] | [[Category: Sullivan, M.]] | ||
[[Category: | [[Category: Actin-binding protein]] | ||
[[Category: | [[Category: Calcium]] | ||
[[Category: | [[Category: Calcium-binding]] | ||
[[Category: | [[Category: Cytoskeleton]] | ||
[[Category: | [[Category: Gelsolin]] | ||
[[Category: | [[Category: Pip2]] | ||
[[Category: | [[Category: Severin]] | ||
[[Category: | [[Category: Villin]] | ||
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Revision as of 09:12, 3 May 2008
SEVERIN DOMAIN 2, 1.75 ANGSTROM CRYSTAL STRUCTURE
OverviewOverview
The crystal structure of the F-actin binding domain 2 of severin, the gelsolin homologue from Dictyostelium discoideum, has been determined by multiple isomorphous replacement and refined to 1.75 A resolution. The structure reveals an alpha-helix-beta-sheet sandwich similar to the domains of gelsolin and villin, and contains two cation-binding sites, as observed in other domain 1 and domain 2 homologues. Comparison of the structures of several gelsolin family domains has identified residues that may mediate F-actin binding in gelsolin domain 2 homologues. To assess the involvement of these residues in F-actin binding, three mutants of human gelsolin domain 2 were assayed for F-actin binding activity and thermodynamic stability. Two of the mutants, RRV168AAA and RLK210AAA, demonstrated a lowered affinity for F-actin, indicating a role for those residues in filament binding. Using both structural and biochemical data, we have constructed a model of the gelsolin domain 1-domain 2-F-actin complex. This model highlights a number of interactions that may serve as positive and negative determinants of filament end- and side-binding.
About this StructureAbout this Structure
1SVY is a Single protein structure of sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA.
ReferenceReference
Mapping the functional surface of domain 2 in the gelsolin superfamily., Puius YA, Fedorov EV, Eichinger L, Schleicher M, Almo SC, Biochemistry. 2000 May 9;39(18):5322-31. PMID:10820002 Page seeded by OCA on Sat May 3 09:12:01 2008