1suc: Difference between revisions

Revision as of 09:08, 3 May 2008

CALCIUM-INDEPENDENT SUBTILISIN BY DESIGN

OverviewOverview

A version of subtilisin BPN' lacking the high affinity calcium site (site A) has been produced through genetic engineering methods, and its crystal structure refined at 1.8 A resolution. This protein and the corresponding version containing the calcium A site are described and compared. The deletion of residues 75-83 was made in the context of four site-specific replacements previously shown to stabilize subtilisin. The helix that in wild type is interrupted by the calcium binding loop, is continuous in the deletion mutant, with normal geometry. A few residues adjacent to the loop, principally those that were involved in calcium coordination, are repositioned and/or destabilized by the deletion. Because refolding is greatly facilitated by the absence of the Ca-loop, this protein offers a new vehicle for analysis and dissection of the folding reaction. This is among the largest internal changes to a protein to be described at atomic resolution.

About this StructureAbout this Structure

1SUC is a Single protein structure of sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA.

ReferenceReference

Calcium-independent subtilisin by design., Gallagher T, Bryan P, Gilliland GL, Proteins. 1993 Jun;16(2):205-13. PMID:8332608 Page seeded by OCA on Sat May 3 09:08:41 2008

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OCA

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 [[Image:1suc.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1suc", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span>
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-|GENE=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1suc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1suc OCA], [http://www.ebi.ac.uk/pdbsum/1suc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1suc RCSB]</span>
-}}
 '''CALCIUM-INDEPENDENT SUBTILISIN BY DESIGN'''
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 [[Category: Gallagher, T.]]
 [[Category: Gilliland, G L.]]
-[[Category: hydrolase(serine proteinase)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 09:08:41 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:47:08 2008''