1slv: Difference between revisions

Revision as of 08:51, 3 May 2008

RAT ANIONIC N143H, E151H TRYPSIN COMPLEXED TO A86H ECOTIN; COPPER-BOUND

OverviewOverview

The three-dimensional structures of complexes of trypsin N143H, E151H bound to ecotin A86H are determined at 2.0 A resolution via X-ray crystallography in the absence and presence of the transition metals Zn2+, Ni2+, and Cu2+. The binding site for these transition metals was constructed by substitution of key amino acids with histidine at the trypsin-ecotin interface in the S2'/P2' pocket. Three histidine side chains, two on trypsin at positions 143 and 151 and one on ecotin at position 86, anchor the metals and provide extended catalytic recognition for substrates with His in the P2' pocket. Comparisons of the three-dimensional structures show the different geometries that result upon the binding of metal in the engineered tridentate site and suggest a structural basis for the kinetics of the metal-regulated catalysis. Of the three metals, the binding of zinc results in the most favorable binding geometry, not dissimilar to those observed in naturally occurring zinc binding proteins.

About this StructureAbout this Structure

1SLV is a Protein complex structure of sequences from Escherichia coli and Rattus norvegicus. Full crystallographic information is available from OCA.

ReferenceReference

X-ray structures of a designed binding site in trypsin show metal-dependent geometry., Brinen LS, Willett WS, Craik CS, Fletterick RJ, Biochemistry. 1996 May 14;35(19):5999-6009. PMID:8634241 Page seeded by OCA on Sat May 3 08:51:53 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1slv.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1slv |SIZE=350|CAPTION= <scene name='initialview01'>1slv</scene>, resolution 2.3&Aring;
+The line below this paragraph, containing "STRUCTURE_1slv", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=CU1:Cu+Binding+Site'>CU1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= RAT ANIONIC TRYPSIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), RAT ANIONIC TRYPSIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
+-->
-|DOMAIN=
+{{STRUCTURE_1slv|  PDB=1slv  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1slv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1slv OCA], [http://www.ebi.ac.uk/pdbsum/1slv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1slv RCSB]</span>
-}}
 '''RAT ANIONIC N143H, E151H TRYPSIN COMPLEXED TO A86H ECOTIN; COPPER-BOUND'''
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 [[Category: Brinen, L S.]]
 [[Category: Fletterick, R J.]]
-[[Category: complex]]
+[[Category: Complex]]
-[[Category: inhibitor]]
+[[Category: Inhibitor]]
-[[Category: metal binding site]]
+[[Category: Metal binding site]]
-[[Category: metalloprotein]]
+[[Category: Metalloprotein]]
-[[Category: protease-substrate interaction]]
+[[Category: Protease-substrate interaction]]
-[[Category: protein engineering]]
+[[Category: Protein engineering]]
-[[Category: serine protease]]
+[[Category: Serine protease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:51:53 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:43:50 2008''