4ziz: Difference between revisions
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==Serial Femtosecond Crystallography of Soluble Proteins in Lipidic Cubic Phase (C-Phycocyanin from T. elongatus)== | ==Serial Femtosecond Crystallography of Soluble Proteins in Lipidic Cubic Phase (C-Phycocyanin from T. elongatus)== | ||
<StructureSection load='4ziz' size='340' side='right' caption='[[4ziz]], [[Resolution|resolution]] 1.75Å' scene=''> | <StructureSection load='4ziz' size='340' side='right'caption='[[4ziz]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ziz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus Thermosynechococcus elongatus] and [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_(strain_bp-1) Thermosynechococcus elongatus (strain bp-1)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZIZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZIZ FirstGlance]. <br> | <table><tr><td colspan='2'>[[4ziz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus Thermosynechococcus elongatus] and [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_(strain_bp-1) Thermosynechococcus elongatus (strain bp-1)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZIZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZIZ FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 4ziz" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 4ziz" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Phycocyanin|Phycocyanin]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Thermosynechococcus elongatus]] | [[Category: Thermosynechococcus elongatus]] | ||
[[Category: Barty, A]] | [[Category: Barty, A]] | ||
Revision as of 10:49, 4 March 2020
Serial Femtosecond Crystallography of Soluble Proteins in Lipidic Cubic Phase (C-Phycocyanin from T. elongatus)Serial Femtosecond Crystallography of Soluble Proteins in Lipidic Cubic Phase (C-Phycocyanin from T. elongatus)
Structural highlights
Function[PHCA_THEEB] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex. [PHCB_THEEB] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex. Publication Abstract from PubMedSerial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP-SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP-SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein. Serial femtosecond crystallography of soluble proteins in lipidic cubic phase.,Fromme R, Ishchenko A, Metz M, Chowdhury SR, Basu S, Boutet S, Fromme P, White TA, Barty A, Spence JC, Weierstall U, Liu W, Cherezov V IUCrJ. 2015 Aug 4;2(Pt 5):545-51. doi: 10.1107/S2052252515013160. eCollection, 2015 Sep 1. PMID:26306196[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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