1gtf: Difference between revisions
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[[Image:1gtf. | [[Image:1gtf.jpg|left|200px]]<br /><applet load="1gtf" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1gtf" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1gtf, resolution 1.750Å" /> | caption="1gtf, resolution 1.750Å" /> | ||
'''THE STRUCTURE OF THE TRP RNA-BINDING ATTENUATION PROTEIN (TRAP) BOUND TO A 53-NUCLEOTIDE RNA MOLECULE CONTAINING GAGUU REPEATS'''<br /> | '''THE STRUCTURE OF THE TRP RNA-BINDING ATTENUATION PROTEIN (TRAP) BOUND TO A 53-NUCLEOTIDE RNA MOLECULE CONTAINING GAGUU REPEATS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1GTF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with TRP as [http://en.wikipedia.org/wiki/ligand ligand]. | 1GTF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with TRP as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:TRP Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:TRP Binding Site For Chain B'>AC2</scene>, <scene name='pdbsite=AC3:TRP Binding Site For Chain C'>AC3</scene>, <scene name='pdbsite=AC4:TRP Binding Site For Chain D'>AC4</scene>, <scene name='pdbsite=AC5:TRP Binding Site For Chain E'>AC5</scene>, <scene name='pdbsite=AC6:TRP Binding Site For Chain F'>AC6</scene>, <scene name='pdbsite=AC7:TRP Binding Site For Chain G'>AC7</scene>, <scene name='pdbsite=AC8:TRP Binding Site For Chain H'>AC8</scene>, <scene name='pdbsite=AC9:TRP Binding Site For Chain I'>AC9</scene>, <scene name='pdbsite=BC1:TRP Binding Site For Chain J'>BC1</scene>, <scene name='pdbsite=BC2:TRP Binding Site For Chain K'>BC2</scene>, <scene name='pdbsite=BC3:TRP Binding Site For Chain L'>BC3</scene>, <scene name='pdbsite=BC4:TRP Binding Site For Chain M'>BC4</scene>, <scene name='pdbsite=BC5:TRP Binding Site For Chain N'>BC5</scene>, <scene name='pdbsite=BC6:TRP Binding Site For Chain O'>BC6</scene>, <scene name='pdbsite=BC7:TRP Binding Site For Chain P'>BC7</scene>, <scene name='pdbsite=BC8:TRP Binding Site For Chain Q'>BC8</scene>, <scene name='pdbsite=BC9:TRP Binding Site For Chain R'>BC9</scene>, <scene name='pdbsite=CC1:TRP Binding Site For Chain S'>CC1</scene>, <scene name='pdbsite=CC2:TRP Binding Site For Chain T'>CC2</scene>, <scene name='pdbsite=CC3:TRP Binding Site For Chain U'>CC3</scene> and <scene name='pdbsite=CC4:TRP Binding Site For Chain V'>CC4</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GTF OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: trp rna]] | [[Category: trp rna]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:35:40 2007'' | ||
Revision as of 16:25, 18 December 2007
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THE STRUCTURE OF THE TRP RNA-BINDING ATTENUATION PROTEIN (TRAP) BOUND TO A 53-NUCLEOTIDE RNA MOLECULE CONTAINING GAGUU REPEATS
OverviewOverview
The trp RNA-binding attenuation protein (TRAP) regulates expression of the, tryptophan biosynthetic genes in bacilli by binding to the leader region, of the nascent trp operon mRNA. When activated by binding tryptophan, the, 11-subunit circular TRAP molecule binds to a target sequence consisting of, 11 (G/U)AG repeats, separated by two or three variable 'spacer', nucleotides. Reported here are two crystal structures of TRAP bound to, RNAs containing 11 GAG repeats separated by UU and CC spacer nucleotides, determined at 1.75 and 2.50 A resolution, respectively. These show the, spacer regions of the RNA molecules to be highly flexible, making no, direct hydrogen-bonding contacts with the protein. Comparison of these, structures with the previous structure of TRAP bound to (GAGAU)(10)GAG, RNA, in which the spacer nucleotides stack with each other close to the, protein surface, shows that the RNA can adopt different conformations, depending on the sequence of the spacer regions. This gives insight into, the structural basis of the specificity of TRAP and into the mechanism of, binding.
About this StructureAbout this Structure
1GTF is a Single protein structure of sequence from Geobacillus stearothermophilus with TRP as ligand. Known structural/functional Sites: , , , , , , , , , , , , , , , , , , , , and . Full crystallographic information is available from OCA.
ReferenceReference
Specificity of TRAP-RNA interactions: crystal structures of two complexes with different RNA sequences., Hopcroft NH, Wendt AL, Gollnick P, Antson AA, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):615-21. Epub 2002, Mar 22. PMID:11914485
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