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'''Crystal structure of botulinum neurotoxin type B at pH 6.0''' | '''Crystal structure of botulinum neurotoxin type B at pH 6.0''' | ||
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[[Category: Kumaran, D.]] | [[Category: Kumaran, D.]] | ||
[[Category: Swaminathan, S.]] | [[Category: Swaminathan, S.]] | ||
[[Category: | [[Category: Botulinum]] | ||
[[Category: | [[Category: Metal]] | ||
[[Category: | [[Category: Neurotoxin]] | ||
[[Category: | [[Category: Ph]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:08:27 2008'' | |||
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Revision as of 08:08, 3 May 2008
Crystal structure of botulinum neurotoxin type B at pH 6.0
OverviewOverview
Clostridium botulinum neurotoxins are the most potent toxins to humans and cause paralysis by blocking neurotransmitter release at the presynaptic nerve terminals. The toxicity involves four steps, viz., binding to neuronal cells, internalization, translocation, and catalytic activity. While the catalytic activity is a zinc endopeptidase activity on the SNARE complex proteins, the translocation is believed to be a pH-dependent process allowing the translocation domain to change its conformation to penetrate the endosomal membrane. Here, we report the crystal structures of botulinum neurotoxin type B at various pHs and of an apo form of the neurotoxin, and discuss the role of metal ions and the effect of pH variation in the biological activity. Except for the perturbation of a few side chains, the conformation of the catalytic domain is unchanged in the zinc-depleted apotoxin, suggesting that zinc's role is catalytic. We have also identified two calcium ions in the molecule and present biochemical evidence to show that they play a role in the translocation of the light chain through the membrane.
About this StructureAbout this Structure
1S0E is a Single protein structure of sequence from Clostridium botulinum. Full crystallographic information is available from OCA.
ReferenceReference
Role of metals in the biological activity of Clostridium botulinum neurotoxins., Eswaramoorthy S, Kumaran D, Keller J, Swaminathan S, Biochemistry. 2004 Mar 2;43(8):2209-16. PMID:14979717 Page seeded by OCA on Sat May 3 08:08:27 2008