1gq2: Difference between revisions
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[[Image:1gq2.gif|left|200px]]<br /> | [[Image:1gq2.gif|left|200px]]<br /><applet load="1gq2" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1gq2" size=" | |||
caption="1gq2, resolution 2.5Å" /> | caption="1gq2, resolution 2.5Å" /> | ||
'''MALIC ENZYME FROM PIGEON LIVER'''<br /> | '''MALIC ENZYME FROM PIGEON LIVER'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1GQ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Columba_livia Columba livia] with OXL, MN, CL, NA and NAP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Malate_dehydrogenase_(oxaloacetate-decarboxylating)_(NADP(+)) Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.40 1.1.1.40] | 1GQ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Columba_livia Columba livia] with <scene name='pdbligand=OXL:'>OXL</scene>, <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=NAP:'>NAP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Malate_dehydrogenase_(oxaloacetate-decarboxylating)_(NADP(+)) Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.40 1.1.1.40] Known structural/functional Site: <scene name='pdbsite=1:Na+Binding+Site+For+Chain+G'>1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GQ2 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: pigeon liver]] | [[Category: pigeon liver]] | ||
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Revision as of 10:41, 3 February 2008
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MALIC ENZYME FROM PIGEON LIVER
OverviewOverview
Malic enzymes are widely distributed in nature, and have important, biological functions. They catalyze the oxidative decarboxylation of, malate to produce pyruvate and CO(2) in the presence of divalent cations, (Mg(2+), Mn(2+)). Most malic enzymes have a clear selectivity for the, dinucleotide cofactor, being able to use either NAD(+) or NADP(+), but not, both. Structural studies of the human mitochondrial NAD(+)-dependent malic, enzyme established that malic enzymes belong to a new class of oxidative, decarboxylases. Here we report the crystal structure of the pigeon, cytosolic NADP(+)-dependent malic enzyme, in a closed form, in a, quaternary complex with NADP(+), Mn(2+), and oxalate. This represents the, first structural information on an NADP(+)-dependent malic enzyme. Despite, the sequence conservation, there are large differences in several regions, of the pigeon enzyme structure compared to the human enzyme. One region of, such differences is at the binding site for the 2'-phosphate group of the, NADP(+) cofactor, which helps define the cofactor selectivity of the, enzymes. Specifically, the structural information suggests Lys362 may have, an important role in the NADP(+) selectivity of the pigeon enzyme, confirming our earlier kinetic observations on the K362A mutant. Our, structural studies also revealed differences in the organization of the, tetramer between the pigeon and the human enzymes, although the pigeon, enzyme still obeys 222 symmetry.
About this StructureAbout this Structure
1GQ2 is a Single protein structure of sequence from Columba livia with , , , and as ligands. Active as Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)), with EC number 1.1.1.40 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structural studies of the pigeon cytosolic NADP(+)-dependent malic enzyme., Yang Z, Zhang H, Hung HC, Kuo CC, Tsai LC, Yuan HS, Chou WY, Chang GG, Tong L, Protein Sci. 2002 Feb;11(2):332-41. PMID:11790843
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