1rl4: Difference between revisions

Revision as of 07:37, 3 May 2008

Plasmodium falciparum peptide deformylase complex with inhibitor

OverviewOverview

An altered version of peptide deformylase from Plasmodium falciparum (PfPDF), the organism that causes the most devastating form of malaria, has been cocrystallized with a synthesized inhibitor that has submicromolar affinity for its target protein. The structure is solved at 2.2 A resolution, an improvement over the 2.8 A resolution achieved during the structural determination of unliganded PfPDF. This represents the successful outcome of modifying the protein construct in order to overcome adverse crystal contacts and other problems encountered in the study of unliganded PfPDF. Two molecules of PfPDF are found in the asymmetric unit of the current structure. The active site of each monomer of PfPDF is occupied by a proteolyzed fragment of the tripeptide-like inhibitor. Unexpectedly, each PfPDF subunit is associated with two nearly complete molecules of the inhibitor, found at a protein-protein interface. This is the first structure of a eukaryotic PDF protein, a potential drug target, in complex with a ligand.

About this StructureAbout this Structure

1RL4 is a Single protein structure of sequence from Plasmodium falciparum 3d7. Full crystallographic information is available from OCA.

ReferenceReference

An improved crystal form of Plasmodium falciparum peptide deformylase., Robien MA, Nguyen KT, Kumar A, Hirsh I, Turley S, Pei D, Hol WG, Protein Sci. 2004 Apr;13(4):1155-63. Epub 2004 Mar 9. PMID:15010544 Page seeded by OCA on Sat May 3 07:37:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1rl4.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1rl4 |SIZE=350|CAPTION= <scene name='initialview01'>1rl4</scene>, resolution 2.18&Aring;
+The line below this paragraph, containing "STRUCTURE_1rl4", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BL5:2-{N&#39;-[2-(5-AMINO-1-PHENYLCARBAMOYL-PENTYLCARBAMOYL)-HEXYL]-HYDRAZINOMETHYL}-HEXANOIC+ACID(5-AMINO-1-PHENYLCARBAMOYL-PENTYL)-AMIDE'>BL5</scene>, <scene name='pdbligand=BRR:(2R)-2-{[FORMYL(HYDROXY)AMINO]METHYL}HEXANOIC+ACID'>BRR</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Formylmethionine_deformylase Formylmethionine deformylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.31 3.5.1.31] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= PDF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 Plasmodium falciparum 3D7])
+-->
-|DOMAIN=
+{{STRUCTURE_1rl4|  PDB=1rl4  |  SCENE=  }}
-|RELATEDENTRY=[[1jym|1JYM]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rl4 OCA], [http://www.ebi.ac.uk/pdbsum/1rl4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rl4 RCSB]</span>
-}}
 '''Plasmodium falciparum peptide deformylase complex with inhibitor'''
@@ Line 33: / Line 30: @@
 [[Category: Robien, M A.]]
 [[Category: Turley, S.]]
-[[Category: crystal engineering]]
+[[Category: Crystal engineering]]
-[[Category: drug design]]
+[[Category: Drug design]]
-[[Category: malaria]]
+[[Category: Malaria]]
-[[Category: pdf]]
+[[Category: Pdf]]
-[[Category: peptide deformylase]]
+[[Category: Peptide deformylase]]
-[[Category: plasmodium]]
+[[Category: Plasmodium]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:37:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:29:43 2008''