1rio: Difference between revisions

Revision as of 07:32, 3 May 2008

Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA

OverviewOverview

The cI protein of bacteriophage lambda (lambdacI) activates transcription by binding a DNA operator just upstream of the promoter and interacting with the RNA polymerase sigma subunit domain 4 (sigma(4)). We determined the crystal structure of the lambdacI/sigma(4)/DNA ternary complex at 2.3 A resolution. There are no conformational changes in either protein, which interact through an extremely small interface involving at most 6 amino acid residues. The interactions of the two proteins stabilize the binding of each protein to the DNA. The results provide insight into how activators can operate through a simple cooperative binding mechanism but affect different steps of the transcription initiation process.

About this StructureAbout this Structure

1RIO is a Protein complex structure of sequences from Enterobacteria phage lambda and Thermus aquaticus. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a ternary transcription activation complex., Jain D, Nickels BE, Sun L, Hochschild A, Darst SA, Mol Cell. 2004 Jan 16;13(1):45-53. PMID:14731393 Page seeded by OCA on Sat May 3 07:32:44 2008

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OCA

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 [[Image:1rio.gif|left|200px]]
- {{Structure
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-|GENE= CI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda])
+-->
-|DOMAIN=
+{{STRUCTURE_1rio|  PDB=1rio  |  SCENE=  }}
-|RELATEDENTRY=[[1ku7|1KU7]], [[1lmb|1LMB]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rio OCA], [http://www.ebi.ac.uk/pdbsum/1rio PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rio RCSB]</span>
-}}
 '''Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA'''
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 [[Category: Nickels, B E.]]
 [[Category: Sun, L.]]
-[[Category: helix-turn-helix]]
+[[Category: Helix-turn-helix]]
-[[Category: transcription activation]]
+[[Category: Transcription activation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:32:44 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:28:40 2008''