1r0f: Difference between revisions
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'''Gallium-substituted rubredoxin''' | '''Gallium-substituted rubredoxin''' | ||
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[[Category: Wedd, A G.]] | [[Category: Wedd, A G.]] | ||
[[Category: Wilce, M C.J.]] | [[Category: Wilce, M C.J.]] | ||
[[Category: | [[Category: Clostridium pasteurianum]] | ||
[[Category: | [[Category: Iron-sulfur]] | ||
[[Category: | [[Category: Rubredoxin]] | ||
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Revision as of 06:55, 3 May 2008
Gallium-substituted rubredoxin
OverviewOverview
Five different metal-substituted forms of Clostridium pasteurianum rubredoxin have been prepared and crystallized. The single Fe atom present in the Fe(S-Cys)(4) site of the native form of the protein was exchanged in turn for Co, Ni, Ga, Cd and Hg. All five forms of rubredoxin crystallized in space group R3 and were isomorphous with the native protein. The Co-, Ni- and Ga-substituted proteins exhibited metal sites with geometries similar to that of the Fe form (effective D(2d) local symmetry), as did the Cd and Hg proteins, but with a significant expansion of the metal-sulfur bond lengths. A knowledge of these structures contributes to a molecular understanding of the function of this simple iron-sulfur electron-transport protein.
About this StructureAbout this Structure
1R0F is a Single protein structure of sequence from Clostridium pasteurianum. Full crystallographic information is available from OCA.
ReferenceReference
Metal-substituted derivatives of the rubredoxin from Clostridium pasteurianum., Maher M, Cross M, Wilce MC, Guss JM, Wedd AG, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):298-303. Epub 2004, Jan 23. PMID:14747706 Page seeded by OCA on Sat May 3 06:55:16 2008