1r0c: Difference between revisions

Revision as of 06:55, 3 May 2008

Products in the T State of Aspartate Transcarbamylase: Crystal Structure of the Phosphate and N-carbamyl-L-aspartate Ligated Enzyme

OverviewOverview

The structure of aspartate transcarbamylase of Escherichia coli ligated to products (phosphate and N-carbamyl-l-aspartate) has been determined at 2.37 A resolution (R-factor = 0.23, R(free) = 0.27). Results might indicate a product release mode, rather than close analogues to the transition state like those found in our earlier studies of other ligands (N-phosphonacetyl-L-aspartate, carbamyl phosphate plus malonate, phosphonoacetamide plus malonate, or citrate plus phosphate). Ordered product release, first carbamylaspartate (CLA) and then phosphate, might be facilitated by a 4 A movement of phosphate from the substrate-analogue position to the product (phosphate) binding position, and by a somewhat similar release movement of the other product (CLA) relative to its analogue (citrate). This movement is consistent with earlier studies of binding of either pyrophosphate or phosphate alone [Honzatko, R. B., and Lipscomb, W. N. (1982) J. Mol. Biol. 160, 265-286].

About this StructureAbout this Structure

1R0C is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Products in the T-state of aspartate transcarbamylase: crystal structure of the phosphate and N-carbamyl-L-aspartate ligated enzyme., Huang J, Lipscomb WN, Biochemistry. 2004 Jun 1;43(21):6422-6. PMID:15157076 Page seeded by OCA on Sat May 3 06:55:08 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1r0c.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1r0c |SIZE=350|CAPTION= <scene name='initialview01'>1r0c</scene>, resolution 2.37&Aring;
+The line below this paragraph, containing "STRUCTURE_1r0c", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=NCD:N-CARBAMOYL-L-ASPARTATE'>NCD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= PYRB OR B4245 OR C5345 OR Z5856 OR ECS5222 OR SF4245 OR S4507 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), PYRI OR B4244 OR C5344 OR Z5855 OR ECS5221 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_1r0c|  PDB=1r0c  |  SCENE=  }}
-|RELATEDENTRY=[[1r0b|1R0B]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r0c OCA], [http://www.ebi.ac.uk/pdbsum/1r0c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r0c RCSB]</span>
-}}
 '''Products in the T State of Aspartate Transcarbamylase: Crystal Structure of the Phosphate and N-carbamyl-L-aspartate Ligated Enzyme'''
@@ Line 28: / Line 25: @@
 [[Category: Huang, J.]]
 [[Category: Lipscomb, W N.]]
-[[Category: aspartate carbamoyltransferase]]
+[[Category: Aspartate carbamoyltransferase]]
-[[Category: aspartate transcarbamylase]]
+[[Category: Aspartate transcarbamylase]]
-[[Category: atcase-products complex]]
+[[Category: Atcase-products complex]]
-[[Category: n-carbamyl-l-aspartate(cla)]]
+[[Category: Phosphate]]
-[[Category: phosphate]]
+[[Category: Product]]
-[[Category: product]]
+[[Category: T state]]
-[[Category: t state]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:55:08 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:21:27 2008''