1qzc: Difference between revisions

Revision as of 06:53, 3 May 2008

Coordinates of S12, SH44, LH69 and SRL separately fitted into the cryo-EM map of EF-Tu ternary complex (GDP.Kirromycin) bound 70S ribosome

OverviewOverview

Aminoacyl-tRNAs (aa-tRNAs) are delivered to the ribosome as part of the ternary complex of aa-tRNA, elongation factor Tu (EF-Tu) and GTP. Here, we present a cryo-electron microscopy (cryo-EM) study, at a resolution of approximately 9 A, showing that during the incorporation of the aa-tRNA into the 70S ribosome of Escherichia coli, the flexibility of aa-tRNA allows the initial codon recognition and its accommodation into the ribosomal A site. In addition, a conformational change observed in the GTPase-associated center (GAC) of the ribosomal 50S subunit may provide the mechanism by which the ribosome promotes a relative movement of the aa-tRNA with respect to EF-Tu. This relative rearrangement seems to facilitate codon recognition by the incoming aa-tRNA, and to provide the codon-anticodon recognition-dependent signal for the GTPase activity of EF-Tu. From these new findings we propose a mechanism that can explain the sequence of events during the decoding of mRNA on the ribosome.

About this StructureAbout this Structure

1QZC is a Single protein structure of sequence from Escherichia coli and Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy., Valle M, Zavialov A, Li W, Stagg SM, Sengupta J, Nielsen RC, Nissen P, Harvey SC, Ehrenberg M, Frank J, Nat Struct Biol. 2003 Nov;10(11):899-906. Epub 2003 Oct 19. PMID:14566331 Page seeded by OCA on Sat May 3 06:53:20 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1qzc.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1qzc", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|DOMAIN=
+{{STRUCTURE_1qzc|  PDB=1qzc  |  SCENE=  }}
-|RELATEDENTRY=[[1ffk|1FFK]], [[1ibm|1IBM]], [[1qza|1QZA]], [[1qzb|1QZB]], [[1qzd|1QZD]], [[1r2w|1R2W]], [[1r2x|1R2X]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzc OCA], [http://www.ebi.ac.uk/pdbsum/1qzc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qzc RCSB]</span>
-}}
 '''Coordinates of S12, SH44, LH69 and SRL separately fitted into the cryo-EM map of EF-Tu ternary complex (GDP.Kirromycin) bound 70S ribosome'''
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 [[Category: Valle, M.]]
 [[Category: Zavialov, A.]]
-[[Category: ribosomal protein]]
+[[Category: Ribosomal protein]]
-[[Category: rrna]]
+[[Category: Rrna]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:53:20 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:21:07 2008''