3pg1: Difference between revisions
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==MAP kinase LmaMPK10 from Leishmania major (1.95 angs resolution)== | ==MAP kinase LmaMPK10 from Leishmania major (1.95 angs resolution)== | ||
<StructureSection load='3pg1' size='340' side='right' caption='[[3pg1]], [[Resolution|resolution]] 1.95Å' scene=''> | <StructureSection load='3pg1' size='340' side='right'caption='[[3pg1]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3pg1]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3pg1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leima Leima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PG1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PG1 FirstGlance]. <br> | ||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LmjF10.0200 ([ | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LmjF10.0200 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5664 LEIMA])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pg1 OCA], [https://pdbe.org/3pg1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pg1 RCSB], [https://www.ebi.ac.uk/pdbsum/3pg1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pg1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Leima]] | [[Category: Leima]] | ||
[[Category: Buschiazzo, A]] | [[Category: Buschiazzo, A]] | ||
Revision as of 11:37, 25 May 2022
MAP kinase LmaMPK10 from Leishmania major (1.95 angs resolution)MAP kinase LmaMPK10 from Leishmania major (1.95 angs resolution)
Structural highlights
Publication Abstract from PubMedMitogen-activated protein kinases (MAPKs) are involved in environmental signal sensing. They are thus expected to play key roles in the biology of Trypanosomatid parasites, which display complex life cycles and use extracellular cues to modulate cell differentiation. Despite their relevance, structural data of Trypanosomatid MAPKs is lacking. We have now determined the crystal structure of Leishmania major LmaMPK10, a stage-specifically activated MAPK, both alone and in complex with SB203580. LmaMPK10 was observed to be more similar to p38 than to other human MAPKs. However, significant differences could be identified in the catalytic pocket, as well as in potentially regulatory sites in the N-terminal lobe. The modified pocket architecture in LmaMPK10 precludes DFG-in/DFG-out regulatory flipping as observed in mammalian MAPKs. LmaMPK10-nucleotide association was also studied, revealing a potential C-terminal autoinhibitory mechanism. Overall, these data should speed the discovery of molecules interfering with LmaMPK10 functions, with relevance for antileishmanial drug development strategies. The Crystal Structure of the MAP Kinase LmaMPK10 from Leishmania Major Reveals Parasite-Specific Features and Regulatory Mechanisms.,Horjales S, Schmidt-Arras D, Limardo RR, Leclercq O, Obal G, Prina E, Turjanski AG, Spath GF, Buschiazzo A Structure. 2012 Aug 7. PMID:22884419[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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