5k2c: Difference between revisions
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<StructureSection load='5k2c' size='340' side='right' caption='[[5k2c]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='5k2c' size='340' side='right' caption='[[5k2c]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5k2c]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K2C FirstGlance]. <br> | <table><tr><td colspan='2'>[[5k2c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K2C FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZMA:4-{2-[(7-AMINO-2-FURAN-2-YL[1,2,4]TRIAZOLO[1,5-A][1,3,5]TRIAZIN-5-YL)AMINO]ETHYL}PHENOL'>ZMA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZMA:4-{2-[(7-AMINO-2-FURAN-2-YL[1,2,4]TRIAZOLO[1,5-A][1,3,5]TRIAZIN-5-YL)AMINO]ETHYL}PHENOL'>ZMA</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5k2a|5k2a]], [[5k2b|5k2b]], [[5k2d|5k2d]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5k2a|5k2a]], [[5k2b|5k2b]], [[5k2d|5k2d]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ADORA2A, ADORA2, cybC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k2c OCA], [http://pdbe.org/5k2c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k2c RCSB], [http://www.ebi.ac.uk/pdbsum/5k2c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k2c ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k2c OCA], [http://pdbe.org/5k2c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k2c RCSB], [http://www.ebi.ac.uk/pdbsum/5k2c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k2c ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5k2c" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5k2c" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bacillus coli migula 1895]] | |||
[[Category: Aquila, A]] | [[Category: Aquila, A]] | ||
[[Category: Barty, A]] | [[Category: Barty, A]] | ||
Revision as of 13:04, 22 November 2017
1.9 angstrom A2a adenosine receptor structure with sulfur SAD phasing and phase extension using XFEL data1.9 angstrom A2a adenosine receptor structure with sulfur SAD phasing and phase extension using XFEL data
Structural highlights
Function[AA2AR_HUMAN] Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Publication Abstract from PubMedSerial femtosecond crystallography (SFX) takes advantage of extremely bright and ultrashort pulses produced by x-ray free-electron lasers (XFELs), allowing for the collection of high-resolution diffraction intensities from micrometer-sized crystals at room temperature with minimal radiation damage, using the principle of "diffraction-before-destruction." However, de novo structure factor phase determination using XFELs has been difficult so far. We demonstrate the ability to solve the crystallographic phase problem for SFX data collected with an XFEL using the anomalous signal from native sulfur atoms, leading to a bias-free room temperature structure of the human A2A adenosine receptor at 1.9 A resolution. The advancement was made possible by recent improvements in SFX data analysis and the design of injectors and delivery media for streaming hydrated microcrystals. This general method should accelerate structural studies of novel difficult-to-crystallize macromolecules and their complexes. Native phasing of x-ray free-electron laser data for a G protein-coupled receptor.,Batyuk A, Galli L, Ishchenko A, Han GW, Gati C, Popov PA, Lee MY, Stauch B, White TA, Barty A, Aquila A, Hunter MS, Liang M, Boutet S, Pu M, Liu ZJ, Nelson G, James D, Li C, Zhao Y, Spence JC, Liu W, Fromme P, Katritch V, Weierstall U, Stevens RC, Cherezov V Sci Adv. 2016 Sep 23;2(9):e1600292. eCollection 2016 Sep. PMID:27679816[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Bacillus coli migula 1895
- Aquila, A
- Barty, A
- Batyuk, A
- Boutet, S
- Cherezov, V
- Fromme, P
- GPCR, GPCR Network
- Galli, L
- Gati, C
- Han, G W
- Hunter, M S
- Ishchenko, A
- James, D
- Katritch, V
- Lee, M Y
- Li, C
- Liang, M
- Liu, W
- Liu, Z J
- Nelson, G
- Popov, P
- Pu, M
- Spence, J C.H
- Stauch, B
- Stevens, R C
- Weierstall, U
- White, T A
- Zhao, Y
- A2a adenosine receptor
- Gpcr
- Gpcr network
- Membrane protein
- Psi-biology
- Structural genomic
- Sulfur sad