1qlg: Difference between revisions

Revision as of 06:24, 3 May 2008

CRYSTAL STRUCTURE OF PHYTASE WITH MAGNESIUM FROM BACILLUS AMYLOLIQUEFACIENS

OverviewOverview

Phytases hydrolyze phytic acid to less phosphorylated myo-inositol derivatives and inorganic phosphate. A thermostable phytase is of great value in applications for improving phosphate and metal ion availability in animal feed, and thereby reducing phosphate pollution to the environment. Here, we report a new folding architecture of a six-bladed propeller for phosphatase activity revealed by the 2.1 A crystal structures of a novel, thermostable phytase determined in both the partially and fully Ca2+-loaded states. Binding of two calcium ions to high-affinity calcium binding sites results in a dramatic increase in thermostability (by as much as approximately 30 degrees C in melting temperature) by joining loop segments remote in the amino acid sequence. Binding of three additional calcium ions to low-affinity calcium binding sites at the top of the molecule turns on the catalytic activity of the enzyme by converting the highly negatively charged cleft into a favorable environment for the binding of phytate.

About this StructureAbout this Structure

1QLG is a Single protein structure of sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of a novel, thermostable phytase in partially and fully calcium-loaded states., Ha NC, Oh BC, Shin S, Kim HJ, Oh TK, Kim YO, Choi KY, Oh BH, Nat Struct Biol. 2000 Feb;7(2):147-53. PMID:10655618 Page seeded by OCA on Sat May 3 06:24:56 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1qlg.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1qlg |SIZE=350|CAPTION= <scene name='initialview01'>1qlg</scene>, resolution 2.2&Aring;
+The line below this paragraph, containing "STRUCTURE_1qlg", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=MG:Mg'>MG</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1qlg|  PDB=1qlg  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qlg OCA], [http://www.ebi.ac.uk/pdbsum/1qlg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qlg RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF PHYTASE WITH MAGNESIUM FROM BACILLUS AMYLOLIQUEFACIENS'''
@@ Line 29: / Line 26: @@
 [[Category: Oh, B H.]]
 [[Category: Shin, S.]]
-[[Category: calcium]]
+[[Category: Calcium]]
-[[Category: magnesium]]
+[[Category: Magnesium]]
-[[Category: phosphatase]]
+[[Category: Phosphatase]]
-[[Category: phytase]]
+[[Category: Phytase]]
-[[Category: thermostable]]
+[[Category: Thermostable]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:24:56 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:15:26 2008''