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{{STRUCTURE_1q3v| PDB=1q3v | SCENE= }} | |||
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'''Crystal structure of a wild-type Cre recombinase-loxP synapse: phosphotyrosine covalent intermediate''' | '''Crystal structure of a wild-type Cre recombinase-loxP synapse: phosphotyrosine covalent intermediate''' | ||
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[[Category: Stewart, A F.]] | [[Category: Stewart, A F.]] | ||
[[Category: Suck, D.]] | [[Category: Suck, D.]] | ||
[[Category: | [[Category: Cre]] | ||
[[Category: | [[Category: Dna]] | ||
[[Category: | [[Category: Recombinase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:50:07 2008'' | |||
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Revision as of 05:50, 3 May 2008
Crystal structure of a wild-type Cre recombinase-loxP synapse: phosphotyrosine covalent intermediate
OverviewOverview
Escherichia coli phage P1 Cre recombinase catalyzes the site-specific recombination of DNA containing loxP sites. We report here two crystal structures of a wild-type Cre recombinase-loxP synaptic complex corresponding to two distinct reaction states: an initial pre-cleavage complex, trapped using a phosphorothioate modification at the cleavable scissile bond that prevents the recombination reaction, and a 3'-phosphotyrosine protein-DNA intermediate resulting from the first strand cleavage. In contrast to previously determined Cre complexes, both structures contain a full tetrameric complex in the asymmetric unit, unequivocally showing that the anti-parallel arrangement of the loxP sites is an intrinsic property of the Cre-loxP recombination synapse. The conformation of the spacer is different to the one observed for the symmetrized loxS site: a kink next to the scissile phosphate in the top strand of the pre-cleavage complex leads to unstacking of the TpG step and a widening of the minor groove. This side of the spacer is interacting with a 'cleavage-competent' Cre subunit, suggesting that the first cleavage occurs at the ApT step in the top strand. This is further confirmed by the structure of the 3'-phosphotyrosine intermediate, where the DNA is cleaved in the top strands and covalently linked to the 'cleavage-competent' subunits. The cleavage is followed by a movement of the C-terminal part containing the attacking Y324 and the helix N interacting with the 'non-cleaving' subunit. This rearrangement could be responsible for the interconversion of Cre subunits. Our results also suggest that the Cre-induced kink next to the scissile phosphodiester activates the DNA for cleavage at this position and facilitates strand transfer.
About this StructureAbout this Structure
1Q3V is a Single protein structure of sequence from Enterobacteria phage p1. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a wild-type Cre recombinase-loxP synapse reveals a novel spacer conformation suggesting an alternative mechanism for DNA cleavage activation., Ennifar E, Meyer JE, Buchholz F, Stewart AF, Suck D, Nucleic Acids Res. 2003 Sep 15;31(18):5449-60. PMID:12954782 Page seeded by OCA on Sat May 3 05:50:07 2008