1py0: Difference between revisions

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Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe

OverviewOverview

A lanthanide complex, named CLaNP (caged lanthanide NMR probe) has been developed for the characterisation of proteins by paramagnetic NMR spectroscopy. The probe consists of a lanthanide chelated by a derivative of DTPA (diethylenetriaminepentaacetic acid) with two thiol reactive functional groups. The CLaNP molecule is attached to a protein by two engineered, surface-exposed, Cys residues in a bidentate manner. This drastically limits the dynamics of the metal relative to the protein and enables measurements of pseudocontact shifts. NMR spectroscopy experiments on a diamagnetic control and the crystal structure of the probe-protein complex demonstrate that the protein structure is not affected by probe attachment. The probe is able to induce pseudocontact shifts to at least 40 A from the metal and causes residual dipolar couplings due to alignment at a high magnetic field. The molecule exists in several isomeric forms with different paramagnetic tensors; this provides a fast way to obtain long-range distance restraints.

About this StructureAbout this Structure

1PY0 is a Single protein structure of sequence from Alcaligenes faecalis. Full crystallographic information is available from OCA.

ReferenceReference

A caged lanthanide complex as a paramagnetic shift agent for protein NMR., Prudencio M, Rohovec J, Peters JA, Tocheva E, Boulanger MJ, Murphy ME, Hupkes HJ, Kosters W, Impagliazzo A, Ubbink M, Chemistry. 2004 Jul 5;10(13):3252-60. PMID:15224334 Page seeded by OCA on Sat May 3 05:37:46 2008

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OCA

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 '''Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe'''
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 [[Category: Tocheva, E.]]
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-[[Category: cupredoxin]]
+[[Category: Cupredoxin]]
-[[Category: nmr probe]]
+[[Category: Nmr probe]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:37:46 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:06:05 2008''