1peu: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1peu.jpg|left|200px]] | [[Image:1peu.jpg|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1peu", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_1peu| PDB=1peu | SCENE= }} | |||
}} | |||
'''Ribonucleotide Reductase Protein R1E from Salmonella typhimurium''' | '''Ribonucleotide Reductase Protein R1E from Salmonella typhimurium''' | ||
| Line 33: | Line 30: | ||
[[Category: Uppsten, M.]] | [[Category: Uppsten, M.]] | ||
[[Category: 10 stranded alpha/beta barrel]] | [[Category: 10 stranded alpha/beta barrel]] | ||
[[Category: | [[Category: Datp]] | ||
[[Category: | [[Category: Protein-specificity-effector complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:00:24 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 05:00, 3 May 2008
Ribonucleotide Reductase Protein R1E from Salmonella typhimurium
OverviewOverview
The three-dimensional structure of the large subunit of the first member of a class Ib ribonucleotide reductase, R1E of Salmonella typhimurium, has been determined in its native form and together with three allosteric effectors. The enzyme contains the characteristic ten-stranded alpha/beta-barrel with catalytic residues at a finger loop in its center and with redox-active cysteine residues at two adjacent barrel strands. Structures where the redox-active cysteine residues are in reduced thiol form and in oxidized disulfide form have been determined revealing local structural changes. The R1E enzyme differs from the class Ia enzyme, Escherichia coli R1, by not having an overall allosteric regulation. This is explained from the structure by differences in the N-terminal domain, which is about 50 residues shorter and lacks the overall allosteric binding site. R1E has an allosteric substrate specificity regulation site and the binding site for the nucleotide effectors is located at the dimer interface similarly as for the class Ia enzymes. We have determined the structures of R1E in the absence of effectors and with dTTP, dATP and dCTP bound. The low affinity for ATP at the specificity site is explained by a tyrosine, which hinders nucleotides containing a 2'-OH group to bind.
About this StructureAbout this Structure
1PEU is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the large subunit of class Ib ribonucleotide reductase from Salmonella typhimurium and its complexes with allosteric effectors., Uppsten M, Farnegardh M, Jordan A, Eliasson R, Eklund H, Uhlin U, J Mol Biol. 2003 Jun 27;330(1):87-97. PMID:12818204 Page seeded by OCA on Sat May 3 05:00:24 2008