5gyr: Difference between revisions

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-'''Unreleased structure'''
-The entry 5gyr is ON HOLD  until Paper Publication
+==Tetrameric Allochromatium vinosum cytochrome c'==
+<StructureSection load='5gyr' size='340' side='right' caption='[[5gyr]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5gyr]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GYR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GYR FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bbh|1bbh]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gyr OCA], [http://pdbe.org/5gyr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gyr RCSB], [http://www.ebi.ac.uk/pdbsum/5gyr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gyr ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/CYCP_ALLVD CYCP_ALLVD]] Cytochrome c' is the most widely occurring bacterial c-type cytochrome. Cytochromes c' are high-spin proteins and the heme has no sixth ligand. Their exact function is not known.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The number of artificial protein supramolecules has been increasing; however, control of protein oligomer formation remains challenging. Cytochrome c' from Allochromatium vinosum (AVCP) is a homodimeric protein in its native form, where its protomer exhibits a four-helix bundle structure containing a covalently bound five-coordinate heme as a gas binding site. AVCP exhibits a unique reversible dimer-monomer transition according to the absence and presence of CO. Herein, domain-swapped dimeric AVCP was constructed and utilized to form a tetramer and high-order oligomers. The X-ray crystal structure of oxidized tetrameric AVCP consisted of two monomer subunits and one domain-swapped dimer subunit, which exchanged the region containing helices alphaA and alphaB between protomers. The active site structures of the domain-swapped dimer subunit and monomer subunits in the tetramer were similar to those of the monomer subunits in the native dimer. The subunit-subunit interactions at the interfaces of the domain-swapped dimer and monomer subunits in the tetramer were also similar to the subunit-subunit interaction in the native dimer. Reduced tetrameric AVCP dissociated to a domain-swapped dimer and two monomers upon CO binding. Without monomers, the domain-swapped dimers formed tetramers, hexamers, and higher-order oligomers in the absence of CO, whereas the oligomers dissociated to domain-swapped dimers in the presence of CO, demonstrating that the domain-swapped dimer maintains the CO-induced subunit dissociation behavior of native ACVP. These results suggest that protein oligomer formation may be controlled by utilizing domain swapping for a dimer-monomer transition protein.
-Authors: Yamanaka, M., Hoshizumi, M., Nagao, S., Nakayama, R., Shibata, N., Higuchi, Y., Hirota, S.
+Formation and carbon monoxide-dependent dissociation of Allochromatium vinosum cytochrome c' oligomers using domain-swapped dimers.,Yamanaka M, Hoshizumi M, Nagao S, Nakayama R, Shibata N, Higuchi Y, Hirota S Protein Sci. 2017 Mar;26(3):464-474. doi: 10.1002/pro.3090. Epub 2017 Feb 14. PMID:27883268<ref>PMID:27883268</ref>
-Description: Tetrameric Allochromatium vinosum cytochrome c'
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 5gyr" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Higuchi, Y]]
 [[Category: Hirota, S]]
-[[Category: Yamanaka, M]]
-[[Category: Higuchi, Y]]
-[[Category: Nakayama, R]]
 [[Category: Hoshizumi, M]]
 [[Category: Nagao, S]]
+[[Category: Nakayama, R]]
 [[Category: Shibata, N]]
+[[Category: Yamanaka, M]]
+[[Category: Electron transport]]