CRISPR-Cas9: Difference between revisions
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The <scene name='74/742625/Cv/10'>two strands of the guide-target hybrid</scene> do not twist around one another in a helix, but instead adopt an underwound ribbon-like structure reminiscent of a ladder. The 5′ and 3′ ends of the curved target strand are ~102Å apart, roughly the length of straight B-form dsDNA with an identical sequence (~107 Å). The crRNA (green) and ssDNA target (orange) are displayed in a spheres representation. Underwinding is facilitated by <scene name='74/742625/Cv/12'>kinks that occur every sixth base pair in the backbone of both strands of the hybrid</scene> (ribbon representation of the crRNA and ssDNA). <scene name='74/742625/Cv/14'>At each kink, complementary nucleotides are rotated ~90°, in opposing directions, from the axis of the duplex</scene>. Disrupted RNA and DNA nucleotides are colored red and blue, respectively. | The <scene name='74/742625/Cv/10'>two strands of the guide-target hybrid</scene> do not twist around one another in a helix, but instead adopt an underwound ribbon-like structure reminiscent of a ladder. The 5′ and 3′ ends of the curved target strand are ~102Å apart, roughly the length of straight B-form dsDNA with an identical sequence (~107 Å). The crRNA (green) and ssDNA target (orange) are displayed in a spheres representation. Underwinding is facilitated by <scene name='74/742625/Cv/12'>kinks that occur every sixth base pair in the backbone of both strands of the hybrid</scene> (ribbon representation of the crRNA and ssDNA). <scene name='74/742625/Cv/14'>At each kink, complementary nucleotides are rotated ~90°, in opposing directions, from the axis of the duplex</scene>. Disrupted RNA and DNA nucleotides are colored red and blue, respectively. | ||
<scene name='74/742625/Cv/16'>Rocket representation of Cas7 colored by domain: thumb (green), fingers (blue), and palm (purple).</scene> | |||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||