1oyv: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1oyv.jpg|left|200px]] | [[Image:1oyv.jpg|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1oyv", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
| | --> | ||
| | {{STRUCTURE_1oyv| PDB=1oyv | SCENE= }} | ||
}} | |||
'''Crystal structure of tomato inhibitor-II in a ternary complex with subtilisin Carlsberg''' | '''Crystal structure of tomato inhibitor-II in a ternary complex with subtilisin Carlsberg''' | ||
| Line 33: | Line 30: | ||
[[Category: Pearce, G.]] | [[Category: Pearce, G.]] | ||
[[Category: Ryan, C A.]] | [[Category: Ryan, C A.]] | ||
[[Category: | [[Category: Multidomain inhibitor]] | ||
[[Category: | [[Category: Potato ii family]] | ||
[[Category: | [[Category: Serine proteinase inhibitor]] | ||
[[Category: | [[Category: Ternary complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:26:51 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 04:26, 3 May 2008
Crystal structure of tomato inhibitor-II in a ternary complex with subtilisin Carlsberg
OverviewOverview
Multidomain proteinase inhibitors play critical roles in the defense of plants against predation by a wide range of pests. Despite a wealth of structural information on proteinase-single domain inhibitor interactions, the structural basis of inhibition by multidomain proteinase inhibitors remains poorly understood. Here we report the 2.5-A resolution crystal structure of the two-headed tomato inhibitor-II (TI-II) in complex with two molecules of subtilisin Carlsberg; it reveals how a multidomain inhibitor from the Potato II family of proteinase inhibitors can bind to and simultaneously inhibit two enzyme molecules within a single ternary complex. The N terminus of TI-II initiates the folding of Domain I (Lys-1 to Cys-15 and Pro-84 to Met-123) and then completes Domain II (Ile-26 to Pro-74) before coming back to complete the rest of Domain I (Pro-84 to Met-123). The two domains of TI-II adopt a similar fold and are arranged in an extended configuration that presents two reactive site loops at the opposite ends of the inhibitor molecule. Each subtilisin molecule interacts with a reactive site loop of TI-II through the standard, canonical binding mode. Remarkably, a significant distortion of the active site of subtilisin is induced by the presence of phenylalanine in the P1 position of reactive site loop II of TI-II. The structure of the TI-II.(subtilisin)2 complex provides a molecular framework for understanding how multiple inhibitory domains in a single Potato II type proteinase inhibitor molecule from the Potato II family act to inhibit proteolytic enzymes.
About this StructureAbout this Structure
1OYV is a Protein complex structure of sequences from Bacillus licheniformis and Solanum lycopersicum. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of inhibition revealed by a 1:2 complex of the two-headed tomato inhibitor-II and subtilisin Carlsberg., Barrette-Ng IH, Ng KK, Cherney MM, Pearce G, Ryan CA, James MN, J Biol Chem. 2003 Jun 27;278(26):24062-71. Epub 2003 Apr 8. PMID:12684499 Page seeded by OCA on Sat May 3 04:26:51 2008