1oy3: Difference between revisions

Revision as of 04:25, 3 May 2008

CRYSTAL STRUCTURE OF AN IKBBETA/NF-KB P65 HOMODIMER COMPLEX

OverviewOverview

We report the crystal structure of a murine IkappaBbeta x NF-kappaB p65 homodimer complex. Crystallographic models were determined for two triclinic crystalline systems and refined against data at 2.5 and 2.1 A. The overall complex structure is similar to that of the IkappaBalpha.NF-kappaB p50/p65 heterodimer complex. One NF-kappaB p65 subunit nuclear localization signal clearly contacts IkappaBbeta, whereas a homologous segment from the second subunit of the homodimer is mostly solvent-exposed. The unique 47-amino acid insertion between ankyrin repeats three and four of IkappaBbeta is mostly disordered in the structure. Primary sequence analysis and differences in the mode of binding at the IkappaBbeta sixth ankyrin repeat and NF-kappaB p65 homodimer suggest a model for nuclear IkappaBbeta.NF-kappaB.DNA ternary complex formation. These unique structural features of IkappaBbeta may contribute to its ability to mediate persistent NF-kappaB activation.

About this StructureAbout this Structure

1OY3 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

X-ray crystal structure of an IkappaBbeta x NF-kappaB p65 homodimer complex., Malek S, Huang DB, Huxford T, Ghosh S, Ghosh G, J Biol Chem. 2003 Jun 20;278(25):23094-100. Epub 2003 Apr 9. PMID:12686541 Page seeded by OCA on Sat May 3 04:25:21 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1oy3.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1oy3 |SIZE=350|CAPTION= <scene name='initialview01'>1oy3</scene>, resolution 2.05&Aring;
+The line below this paragraph, containing "STRUCTURE_1oy3", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= RELA OR NFKB3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
+-->
-|DOMAIN=
+{{STRUCTURE_1oy3|  PDB=1oy3  |  SCENE=  }}
-|RELATEDENTRY=[[1k3z|1K3Z]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oy3 OCA], [http://www.ebi.ac.uk/pdbsum/1oy3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oy3 RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF AN IKBBETA/NF-KB P65 HOMODIMER COMPLEX'''
@@ Line 30: / Line 27: @@
 [[Category: Huxford, T.]]
 [[Category: Malek, S.]]
-[[Category: nuclear localization]]
+[[Category: Nuclear localization]]
-[[Category: protein-protein complex]]
+[[Category: Protein-protein complex]]
-[[Category: transcription factor]]
+[[Category: Transcription factor]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:25:21 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:52:03 2008''