Transposase: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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-<StructureSection load='4fcy' size='340' side='right' caption='Structure of Mu transposase complex with DNA (PDB code [[4fcy]]).' scene=''>
+<StructureSection load='3ecp' size='340' side='right' caption='Structure of Tn5 transposase complex with DNA (PDB code [[3ecp]]).' scene=''>
 == Function ==
-'''Transposases''' bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism<ref>PMID:19478801</ref>.  Transposase contains a DDE motif in its active site enabling it to coordinate the binding of divalent metal ions.  The metal ions affect transposase reactivity.  <br />
+'''Transposases''' bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism<ref>PMID:19478801</ref>.    <br />
 *'''Mu transposase''' (MuA) is essential for integration, replication-transposition and excision of bacteriophage Mu DNA into multiple sites of bacterial genome.  Mu transposition occurs within the transposome which is a protein-DNA complex which includes 4 subunits of MuA<ref>PMID:7628012</ref>.<br />
 *'''Hermes transposase''' is a fly protein<ref>PMID:19450689</ref>.<br />
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 *'''Mos1 transposase''' mediates the movement of Mos1 transposon<ref>PMID:10762422</ref><br />
 *'''Tc3 transposase''' mediates the movement of Tc3 transposon in ''C. elegans''<ref>PMID:7838706</ref><br />
-== Disease ==
-== Relevance ==
 == Structural highlights ==
+Transposase contains a DDE motif in its active site<ref>PMID:18790806</ref> enabling it to coordinate the binding of divalent metal ions.  The metal ions affect transposase reactivity.
 </StructureSection>