1oux: Difference between revisions
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'''LecB (PA-LII) sugar-free''' | '''LecB (PA-LII) sugar-free''' | ||
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[[Category: Tielker, D.]] | [[Category: Tielker, D.]] | ||
[[Category: Wyns, L.]] | [[Category: Wyns, L.]] | ||
[[Category: | [[Category: Carbohydrate]] | ||
[[Category: | [[Category: Lectin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:18:33 2008'' | |||
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Revision as of 04:18, 3 May 2008
LecB (PA-LII) sugar-free
OverviewOverview
The crystal structure of Pseudomonas aeruginosa fucose-specific lectin LecB was determined in its metal-bound and metal-free state as well as in complex with fucose, mannose and fructopyranose. All three monosaccharides bind isosterically via direct interactions with two calcium ions as well as direct hydrogen bonds with several side-chains. The higher affinity for fucose is explained by the details of the binding site around C6 and O1 of fucose. In the mannose and fructose complexes, a carboxylate oxygen atom and one or two hydroxyl groups are partly shielded from solvent upon sugar binding, preventing them from completely fulfilling their hydrogen bonding potential. In the fucose complex, no such defects are observed. Instead, C6 makes favourable interactions with a small hydrophobic patch. Upon demetallization, the C terminus as well as the otherwise rigid metal-binding loop become more mobile and adopt multiple conformations.
About this StructureAbout this Structure
1OUX is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa., Loris R, Tielker D, Jaeger KE, Wyns L, J Mol Biol. 2003 Aug 22;331(4):861-70. PMID:12909014 Page seeded by OCA on Sat May 3 04:18:33 2008