5ki4: Difference between revisions
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==Structural impact of single ribonucleotides in DNA== | ==Structural impact of single ribonucleotides in DNA== | ||
<StructureSection load='5ki4' size='340' side='right' caption='[[5ki4]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | <StructureSection load='5ki4' size='340' side='right'caption='[[5ki4]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5ki4]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KI4 OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[5ki4]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KI4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5KI4 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ki4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ki4 OCA], [http://pdbe.org/5ki4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ki4 RCSB], [http://www.ebi.ac.uk/pdbsum/5ki4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ki4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5ki4" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5ki4" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Reverse transcriptase 3D structures|Reverse transcriptase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Evich, M]] | [[Category: Evich, M]] | ||
[[Category: Spring-Connell, A M]] | [[Category: Spring-Connell, A M]] | ||
[[Category: Storici, F]] | [[Category: Storici, F]] | ||
[[Category: Dna]] | [[Category: Dna]] | ||
Revision as of 18:25, 8 July 2020
Structural impact of single ribonucleotides in DNAStructural impact of single ribonucleotides in DNA
Structural highlights
Publication Abstract from PubMedSingle ribonucleotide intrusions represent the most common non-standard nucleotide in genomic DNA, yet little is known of their structural impact. This lesion incurs genomic instability in addition to affecting the physical properties of the DNA.To probe for structural and dynamic effects of single ribonucleotides in various sequence contexts, AxC, CxG and GxC, where x=rG or dG, we report the structures of three single ribonucleotide containing DNA duplexes and the corresponding DNA controls. This lesion subtly and locally perturbs the structure asymmetrically 3' of the lesion on both the riboguanosine-containing and complementary strand of the duplex. The perturbations are mainly restricted to the sugar and phosphodiester backbone. The ribose and 3' downstream deoxyriboses are predominately in N-type conformation, while backbone torsion angles epsilon and/or zeta of the ribonucleotide or upstream deoxyribonucleotide are affected. Depending on the flanking sequences, the C2'-OH group forms hydrogen bonds with the backbone, 3'- neighboring base and/or sugar. Interestingly, even in a similar purine-rG-pyrimidine environment (A-rG-C and G-rG-C), a riboguanosine affects DNA in a distinct manner and manifests different hydrogen bonds, which makes generalizations difficult. Structural impact of single ribonucleotides in DNA.,Evich M, Spring-Connell AM, Storici F, Germann MW Chembiochem. 2016 Aug 9. doi: 10.1002/cbic.201600385. PMID:27504600[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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