1oo3: Difference between revisions
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'''P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase''' | '''P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase''' | ||
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[[Category: Schaffhausen, B.]] | [[Category: Schaffhausen, B.]] | ||
[[Category: Weyrauch, B.]] | [[Category: Weyrauch, B.]] | ||
[[Category: | [[Category: Src homology 2 domain p85 regulatory subunit mutant]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:05:24 2008'' | |||
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Revision as of 04:05, 3 May 2008
P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase
OverviewOverview
Understanding the specificity of Src homology 2 (SH2) domains is important because of their critical role in cell signaling. Previous genetic analysis has characterized mutants of the N-terminal src homology 2 (SH2) domain of the p85 subunit of phosphoinositide 3-kinase (PI3K). The P395S mutant exhibits a specificity for phosphopeptide binding different from that of the wild-type SH2. The P395S mutant has an increased affinity for the platelet-derived growth factor receptor (PDGFr) compared to polyomavirus middle T antigen (MT). Solution structures of the P395S mutant of the p85 N-SH2 alone and complexed to a PDGFr phosphopeptide were determined to explain the change in specificity. Chemical shift perturbations caused by different peptides were compared for mutant and wild-type structures. The results show that the single P395S mutation has broad effects on the structure. Furthermore, they provide a rationale for the observed changes in binding preference.
About this StructureAbout this Structure
1OO3 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
ReferenceReference
Nuclear magnetic resonance structure of the P395S mutant of the N-SH2 domain of the p85 subunit of PI3 kinase: an SH2 domain with altered specificity., Gunther UL, Weyrauch B, Zhang X, Schaffhausen B, Biochemistry. 2003 Sep 30;42(38):11120-7. PMID:14503862 Page seeded by OCA on Sat May 3 04:05:24 2008