1omr: Difference between revisions
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'''non-myristoylated wild-type bovine recoverin with calcium bound to EF-hand 3''' | '''non-myristoylated wild-type bovine recoverin with calcium bound to EF-hand 3''' | ||
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[[Category: Granzin, J.]] | [[Category: Granzin, J.]] | ||
[[Category: Weiergraber, O H.]] | [[Category: Weiergraber, O H.]] | ||
[[Category: | [[Category: Ef-hand]] | ||
[[Category: | [[Category: Helix-loop-helix]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:02:25 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 04:02, 3 May 2008
non-myristoylated wild-type bovine recoverin with calcium bound to EF-hand 3
OverviewOverview
Recoverin is a Ca2+-regulated signal transduction modulator found in vertebrate retina that has been shown to undergo dramatic conformational changes upon Ca2+ binding to its two functional EF-hand motifs. To elucidate the differential impact of the N-terminal myristoylation as well as occupation of the two Ca2+ binding sites on recoverin structure and function, we have investigated a non-myristoylated E85Q mutant exhibiting virtually no Ca2+ binding to EF-2. Crystal structures of the mutant protein as well as the non-myristoylated wild-type have been determined. Although the non-myristoylated E85Q mutant does not display any functional activity, its three-dimensional structure in the presence of Ca2+ resembles the myristoylated wild-type with two Ca2+ but is quite dissimilar from the myristoylated E85Q mutant. We conclude that the N-terminal myristoyl modification significantly stabilizes the conformation of the Ca2+-free protein (i.e. the T conformation) during the stepwise transition toward the fully Ca2+-occupied state. On the basis of these observations, a refined model for the role of the myristoyl group as an intrinsic allosteric modulator is proposed.
About this StructureAbout this Structure
1OMR is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
ReferenceReference
Impact of N-terminal myristoylation on the Ca2+-dependent conformational transition in recoverin., Weiergraber OH, Senin II, Philippov PP, Granzin J, Koch KW, J Biol Chem. 2003 Jun 20;278(25):22972-9. Epub 2003 Apr 9. PMID:12686556 Page seeded by OCA on Sat May 3 04:02:25 2008