1ok6: Difference between revisions

Revision as of 03:57, 3 May 2008

ORTHORHOMBIC CRYSTAL FORM OF AN ARCHAEAL FRUCTOSE 1,6-BISPHOSPHATE ALDOLASE

OverviewOverview

Fructose-1,6-bisphosphate aldolase (FBPA) catalyzes the reversible cleavage of fructose 1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate in the glycolytic pathway. FBPAs from archaeal organisms have recently been identified and characterized as a divergent family of proteins. Here, we report the first crystal structure of an archaeal FBPA at 1.9-A resolution. The structure of this 280-kDa protein complex was determined using single wavelength anomalous dispersion followed by 10-fold non-crystallographic symmetry averaging and refined to an R-factor of 14.9% (Rfree 17.9%). The protein forms a dimer of pentamers, consisting of subunits adopting the ubiquitous (betaalpha)8 barrel fold. Additionally, a crystal structure of the archaeal FBPA covalently bound to dihydroxyacetone phosphate was solved at 2.1-A resolution. Comparison of the active site residues with those of classical FBPAs, which share no significant sequence identity but display the same overall fold, reveals a common ancestry between these two families of FBPAs. Structural comparisons, furthermore, establish an evolutionary link to the triosephosphate isomerases, a superfamily hitherto considered independent from the superfamily of aldolases.

About this StructureAbout this Structure

1OK6 is a Single protein structure of sequence from Thermoproteus tenax. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of an archaeal class I aldolase and the evolution of (betaalpha)8 barrel proteins., Lorentzen E, Pohl E, Zwart P, Stark A, Russell RB, Knura T, Hensel R, Siebers B, J Biol Chem. 2003 Nov 21;278(47):47253-60. Epub 2003 Aug 26. PMID:12941964 Page seeded by OCA on Sat May 3 03:57:07 2008

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OCA

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 [[Image:1ok6.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ok6 |SIZE=350|CAPTION= <scene name='initialview01'>1ok6</scene>, resolution 2.40&Aring;
+The line below this paragraph, containing "STRUCTURE_1ok6", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1ok6|  PDB=1ok6  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ok6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ok6 OCA], [http://www.ebi.ac.uk/pdbsum/1ok6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ok6 RCSB]</span>
-}}
 '''ORTHORHOMBIC CRYSTAL FORM OF AN ARCHAEAL FRUCTOSE 1,6-BISPHOSPHATE ALDOLASE'''
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 [[Category: Zwart, P.]]
 [[Category: 6-bisphosphate]]
-[[Category: aldolase]]
+[[Category: Aldolase]]
-[[Category: archaeal]]
+[[Category: Archaeal]]
-[[Category: fructose 1]]
+[[Category: Fructose 1]]
-[[Category: glycolytic]]
+[[Category: Glycolytic]]
-[[Category: lyase]]
+[[Category: Lyase]]
-[[Category: orthorhombic]]
+[[Category: Orthorhombic]]
-[[Category: schiff base]]
+[[Category: Schiff base]]
-[[Category: tim barrel]]
+[[Category: Tim barrel]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:57:07 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:46:30 2008''