1oe2: Difference between revisions

Revision as of 03:43, 3 May 2008

ATOMIC RESOLUTION STRUCTURE OF D92E MUTANT OF ALCALIGENES XYLOSOXIDANS NITRITE REDUCTASE

OverviewOverview

We provide the first atomic resolution (<1.20 A) structure of a copper protein, nitrite reductase, and of a mutant of the catalytically important Asp92 residue (D92E). The atomic resolution where carbon-carbon bonds of the peptide become clearly resolved, remains a key goal of structural analysis. Despite much effort and technological progress, still very few structures are known at such resolution. For example, in the Protein Data Bank (PDB) there are some 200 structures of copper proteins but the highest resolution structure is that of amicyanin, a small (12 kDa) protein, which has been resolved to 1.30 A. Here, we present the structures of wild-type copper nitrite reductase (wtNiR) from Alcaligenes xylosoxidans (36.5 kDa monomer), the "half-apo" recombinant native protein and the D92E mutant at 1.04, 1.15 and 1.12A resolutions, respectively. These structures provide the basis from which to build a detailed mechanism of this important enzyme.

About this StructureAbout this Structure

1OE2 is a Single protein structure of sequence from Achromobacter xylosoxidans. Full crystallographic information is available from OCA.

ReferenceReference

Atomic resolution structures of native copper nitrite reductase from Alcaligenes xylosoxidans and the active site mutant Asp92Glu., Ellis MJ, Dodd FE, Sawers G, Eady RR, Hasnain SS, J Mol Biol. 2003 Apr 25;328(2):429-38. PMID:12691751 Page seeded by OCA on Sat May 3 03:43:49 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1oe2.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1oe2 |SIZE=350|CAPTION= <scene name='initialview01'>1oe2</scene>, resolution 1.12&Aring;
+The line below this paragraph, containing "STRUCTURE_1oe2", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Pg4+Binding+Site+For+Chain+A'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|ACTIVITY=
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-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1oe2|  PDB=1oe2  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oe2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oe2 OCA], [http://www.ebi.ac.uk/pdbsum/1oe2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oe2 RCSB]</span>
-}}
 '''ATOMIC RESOLUTION STRUCTURE OF D92E MUTANT OF ALCALIGENES XYLOSOXIDANS NITRITE REDUCTASE'''
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 [[Category: Hasnain, S S.]]
 [[Category: Sawers, G.]]
-[[Category: copper protein]]
+[[Category: Copper protein]]
-[[Category: nitrite reductase]]
+[[Category: Nitrite reductase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:43:49 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:43:54 2008''