4ur0

Crystal structure of the PCE reductive dehalogenase from S. multivorans in complex with trichloroetheneCrystal structure of the PCE reductive dehalogenase from S. multivorans in complex with trichloroethene

Structural highlights 4ur0 is a 2 chain structure with sequence from Sulfurospirillum multivorans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: , , , , Related: 4uqu, 4ur1, 4ur2, 4ur3 Activity: Tetrachloroethene reductive dehalogenase, with EC number 1.97.1.8 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Organohalide-respiring microorganisms can use a variety of persistent pollutants including trichloroethene (TCE) as terminal electron acceptors. The final two-electron transfer step in organohalide respiration is catalyzed by reductive dehalogenases. Here we report the x-ray crystal structure of PceA, an archetypal dehalogenase from Sulfurospirillum multivorans, as well as structures of PceA in complex with TCE and product analogs. The active site harbors a deeply buried norpseudo-B12 cofactor within a nitroreductase fold, also found in a mammalian B12 chaperone. The structures of PceA reveal how a cobalamin supports a reductive haloelimination exploiting a conserved B12-binding scaffold capped by a highly variable substrate-capturing region. Structural basis for organohalide respiration.,Bommer M, Kunze C, Fesseler J, Schubert T, Diekert G, Dobbek H Science. 2014 Oct 2. pii: 1258118. PMID:25278505 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Dehalogenase

spinqualitylabelsall models 4ur0, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image