1ny4: Difference between revisions

Revision as of 03:07, 3 May 2008

Solution structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19.

OverviewOverview

We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The structure, determined rapidly with the aid of automated backbone resonance assignment (AutoAssign) and automated structure determination (AutoStructure) software, is characterized by a four-stranded beta-sheet with a classic Greek-key topology and an oligonucleotide/oligosaccharide beta-barrel (OB) fold. The electrostatic surface of S28E exhibits positive and negative patches on opposite sides, the former constituting a putative binding site for RNA. The 13 C-terminal residues of the protein contain a consensus sequence motif constituting the signature of the S28E protein family. Surprisingly, this C-terminal segment is unstructured in solution.

About this StructureAbout this Structure

1NY4 is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.

ReferenceReference

Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii., Aramini JM, Huang YJ, Cort JR, Goldsmith-Fischman S, Xiao R, Shih LY, Ho CK, Liu J, Rost B, Honig B, Kennedy MA, Acton TB, Montelione GT, Protein Sci. 2003 Dec;12(12):2823-30. PMID:14627742 Page seeded by OCA on Sat May 3 03:07:20 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1ny4.gif|left|200px]]
- {{Structure
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-|GENE= RPS28E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK04007 rps28e]</span>
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ny4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ny4 OCA], [http://www.ebi.ac.uk/pdbsum/1ny4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ny4 RCSB]</span>
-}}
 '''Solution structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19.'''
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 [[Category: Shih, L Y.]]
 [[Category: Xiao, R.]]
-[[Category: autostructure]]
+[[Category: Autostructure]]
-[[Category: jr19]]
+[[Category: Jr19]]
-[[Category: nesg]]
+[[Category: Nesg]]
-[[Category: nmr structure]]
+[[Category: Nmr structure]]
-[[Category: northeast structural genomics consortium]]
+[[Category: Northeast structural genomics consortium]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi]]
+[[Category: Psi]]
-[[Category: ribosomal protein]]
+[[Category: Ribosomal protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:07:20 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:37:07 2008''