1nvt: Difference between revisions

Revision as of 03:02, 3 May 2008

Crystal structure of Shikimate Dehydrogenase (AROE or MJ1084) in complex with NADP+

OverviewOverview

The crystal structure of Methanococcus jannaschii shikimate 5-dehydrogenase (MjSDH) bound to the cofactor nicotinamide adenine dinucleotide phosphate (NADP) has been determined at 2.35 A resolution. Shikimate 5-dehydrogenase (SDH) is responsible for NADP-dependent catalysis of the fourth step in shikimate biosynthesis, which is essential for aromatic amino acid metabolism in bacteria, microbial eukaryotes, and plants. The structure of MjSDH is a compact alpha/beta sandwich with two distinct domains, responsible for binding substrate and the NADP cofactor, respectively. A phylogenetically conserved deep cleft on the protein surface corresponds to the enzyme active site. The structure reveals a topologically new domain fold within the N-terminal segment of the polypeptide chain, which binds substrate and supports dimerization. Insights gained from homology modeling and sequence/structure comparisons suggest that the SDHs represent a unique class of dehydrogenases. The structure provides a framework for further investigation to discover and develop novel inhibitors targeting this essential enzyme.

About this StructureAbout this Structure

1NVT is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of shikimate 5-dehydrogenase (SDH) bound to NADP: insights into function and evolution., Padyana AK, Burley SK, Structure. 2003 Aug;11(8):1005-13. PMID:12906831 Page seeded by OCA on Sat May 3 03:02:27 2008

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OCA

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 [[Image:1nvt.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1nvt", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= aroE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0169 AroE], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam01488 Shikimate_DH], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam08501 Shikimate_dh_N]</span>
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nvt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nvt OCA], [http://www.ebi.ac.uk/pdbsum/1nvt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nvt RCSB]</span>
-}}
 '''Crystal structure of Shikimate Dehydrogenase (AROE or MJ1084) in complex with NADP+'''
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 [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
 [[Category: Padyana, A K.]]
-[[Category: new york structural genomix research consortium]]
+[[Category: New york structural genomix research consortium]]
-[[Category: nysgxrc]]
+[[Category: Nysgxrc]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi]]
+[[Category: Psi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:02:27 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:36:14 2008''