1cuj: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1cuj. | [[Image:1cuj.jpg|left|200px]]<br /><applet load="1cuj" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1cuj" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1cuj, resolution 1.60Å" /> | caption="1cuj, resolution 1.60Å" /> | ||
'''CUTINASE, S120C MUTANT'''<br /> | '''CUTINASE, S120C MUTANT'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
1CUJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] | 1CUJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Known structural/functional Site: <scene name='pdbsite=CAT:Catalytic Triad. In The S120c Structure, SER 120 Has Bee ...'>CAT</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CUJ OCA]. | ||
==Reference== | ==Reference== | ||
| Line 21: | Line 20: | ||
[[Category: serine esterase]] | [[Category: serine esterase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:42:02 2007'' | ||
Revision as of 15:32, 18 December 2007
|
CUTINASE, S120C MUTANT
OverviewOverview
In characterizing mutants and covalently inhibited complexes of Fusarium, solani cutinase, which is a 197-residue lipolytic enzyme, 34 variant, structures, crystallizing in 8 different crystal forms, have been, determined, mostly at high resolution. Taking advantage of this, considerable body of information, a structural comparative analysis was, carried out to investigate the dynamics of cutinase. Surface loops were, identified as the major flexible protein regions, particularly those, forming the active-site groove, whereas the elements constituting the, protein scaffold were found to retain the same conformation in all the, cutinase variants studied. Flexibility turned out to be correlated with, thermal motion. With a given crystal packing environment, a high, flexibility turned out to be correlated with a low involvement in crystal, packing contacts. The high degree of crystal polymorphism, which allowed, different conformations with similar energy to be detected, made it, possible to identify motions which would have remained unidentified if, only a single crystal form had been available. Fairly good agreement was, found to exist between the data obtained from the structural comparison, and those from a molecular dynamics (MD) simulation carried out on the, native enzyme. The crystallographic approach used in this study turned out, to be a suitable tool for investigating cutinase dynamics. Because of the, availability of a set of closely related proteins in different crystal, environments, the intrinsic drawback of a crystallographic approach was, bypassed. By combining several static pictures, the dynamics of the, protein could be monitored much more realistically than what can be, achieved on the basis of static pictures alone.
About this StructureAbout this Structure
1CUJ is a Single protein structure of sequence from Fusarium solani subsp. pisi. Active as Triacylglycerol lipase, with EC number 3.1.1.3 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Dynamics of Fusarium solani cutinase investigated through structural comparison among different crystal forms of its variants., Longhi S, Nicolas A, Creveld L, Egmond M, Verrips CT, de Vlieg J, Martinez C, Cambillau C, Proteins. 1996 Dec;26(4):442-58. PMID:8990497
Page seeded by OCA on Tue Dec 18 14:42:02 2007