3bwp: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of a self-spliced group II intron== | ==Crystal structure of a self-spliced group II intron== | ||
<StructureSection load='3bwp' size='340' side='right' caption='[[3bwp]], [[Resolution|resolution]] 3.10Å' scene=''> | <StructureSection load='3bwp' size='340' side='right'caption='[[3bwp]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3bwp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BWP FirstGlance]. <br> | <table><tr><td colspan='2'>[[3bwp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BWP FirstGlance]. <br> | ||
| Line 20: | Line 20: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Keating, K S]] | [[Category: Keating, K S]] | ||
[[Category: Pyle, A M]] | [[Category: Pyle, A M]] | ||
Revision as of 11:37, 11 March 2020
Crystal structure of a self-spliced group II intronCrystal structure of a self-spliced group II intron
Structural highlights
Publication Abstract from PubMedGroup II introns are self-splicing ribozymes that catalyze their own excision from precursor transcripts and insertion into new genetic locations. Here we report the crystal structure of an intact, self-spliced group II intron from Oceanobacillus iheyensis at 3.1 angstrom resolution. An extensive network of tertiary interactions facilitates the ordered packing of intron subdomains around a ribozyme core that includes catalytic domain V. The bulge of domain V adopts an unusual helical structure that is located adjacent to a major groove triple helix (catalytic triplex). The bulge and catalytic triplex jointly coordinate two divalent metal ions in a configuration that is consistent with a two-metal ion mechanism for catalysis. Structural and functional analogies support the hypothesis that group II introns and the spliceosome share a common ancestor. Crystal structure of a self-spliced group II intron.,Toor N, Keating KS, Taylor SD, Pyle AM Science. 2008 Apr 4;320(5872):77-82. PMID:18388288[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||