3ub7: Difference between revisions
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==Periplasmic portion of the Helicobacter pylori chemoreceptor TlpB with acetamide bound== | ==Periplasmic portion of the Helicobacter pylori chemoreceptor TlpB with acetamide bound== | ||
<StructureSection load='3ub7' size='340' side='right' caption='[[3ub7]], [[Resolution|resolution]] 1.40Å' scene=''> | <StructureSection load='3ub7' size='340' side='right'caption='[[3ub7]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3ub7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori_(strain_ss1) Helicobacter pylori (strain ss1)]. The February 2013 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Proton-Gated Urea Channel'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2013_2 10.2210/rcsb_pdb/mom_2013_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UB7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[3ub7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori_(strain_ss1) Helicobacter pylori (strain ss1)]. The February 2013 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Proton-Gated Urea Channel'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2013_2 10.2210/rcsb_pdb/mom_2013_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UB7 FirstGlance]. <br> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Proton-Gated Urea Channel]] | [[Category: Proton-Gated Urea Channel]] | ||
[[Category: RCSB PDB Molecule of the Month]] | [[Category: RCSB PDB Molecule of the Month]] | ||
Revision as of 20:49, 14 August 2019
Periplasmic portion of the Helicobacter pylori chemoreceptor TlpB with acetamide boundPeriplasmic portion of the Helicobacter pylori chemoreceptor TlpB with acetamide bound
Structural highlights
Publication Abstract from PubMedpH sensing is crucial for survival of most organisms, yet the molecular basis of such sensing is poorly understood. Here, we present an atomic resolution structure of the periplasmic portion of the acid-sensing chemoreceptor, TlpB, from the gastric pathogen Helicobacter pylori. The structure reveals a universal signaling fold, a PAS domain, with a molecule of urea bound with high affinity. Through biophysical, biochemical, and in vivo mutagenesis studies, we show that urea and the urea-binding site residues play critical roles in the ability of H. pylori to sense acid. Our signaling model predicts that protonation events at Asp114, affected by changes in pH, dictate the stability of TlpB through urea binding. Structure and Proposed Mechanism for the pH-Sensing Helicobacter pylori Chemoreceptor TlpB.,Goers Sweeney E, Henderson JN, Goers J, Wreden C, Hicks KG, Foster JK, Parthasarathy R, Remington SJ, Guillemin K Structure. 2012 Jun 14. PMID:22705207[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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