1nqp: Difference between revisions

Revision as of 02:52, 3 May 2008

Crystal structure of Human hemoglobin E at 1.73 A resolution

OverviewOverview

Hemoglobin A(2) (alpha(2)delta(2)), a minor (2-3%) component of circulating red blood cells, acts as an anti-sickling agent and its elevated concentration in beta-thalassemia is a useful clinical diagnostic. In beta-thalassemia major, where there is a failure of beta-chain production, HbA(2) acts as the predominant oxygen delivery mechanism. Hemoglobin E, is another common abnormal hemoglobin, caused by splice site mutation in exon 1 of beta globin gene, when combines with beta-thalassemia, causes severe microcytic anemia. The purification, crystallization, and preliminary structural studies of HbA(2) and HbE are reported here. HbA(2) and HbE are purified by cation exchange column chromatography in presence of KCN from the blood samples of individuals suffering from beta-thalassemia minor and E beta-thalassemia. X-ray diffraction data of HbA(2) and HbE were collected upto 2.1 and 1.73 A, respectively. HbA(2) crystallized in space group P2(1) with unit cell parameters a=54.33 A, b=83.73 A, c=62.87 A, and beta=99.80 degrees whereas HbE crystallized in space group P2(1)2(1)2(1) with unit cell parameters a=60.89 A, b=95.81 A, and c=99.08 A. Asymmetric unit in each case contains one Hb tetramer in R(2) state.

About this StructureAbout this Structure

1NQP is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystallization and preliminary X-ray structural studies of hemoglobin A2 and hemoglobin E, isolated from the blood samples of beta-thalassemic patients., Dasgupta J, Sen U, Choudhury D, Datta P, Chakrabarti A, Chakrabarty SB, Chakrabarty A, Dattagupta JK, Biochem Biophys Res Commun. 2003 Apr 4;303(2):619-23. PMID:12659864 Page seeded by OCA on Sat May 3 02:52:08 2008

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OCA

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 [[Image:1nqp.jpg|left|200px]]
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-}}
 '''Crystal structure of Human hemoglobin E at 1.73 A resolution'''
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 [[Category: Dutta, P.]]
 [[Category: Sen, U.]]
-[[Category: hemoglobin e oxygen transport beta thalassemia]]
+[[Category: Hemoglobin e oxygen transport beta thalassemia]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:52:08 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:34:14 2008''