1noa: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1noa.gif|left|200px]] | [[Image:1noa.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1noa", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
| | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | ||
| | or leave the SCENE parameter empty for the default display. | ||
--> | |||
{{STRUCTURE_1noa| PDB=1noa | SCENE= }} | |||
}} | |||
'''CRYSTAL STRUCTURE OF APO-NEOCARZINOSTATIN AT 0.15 NM RESOLUTION''' | '''CRYSTAL STRUCTURE OF APO-NEOCARZINOSTATIN AT 0.15 NM RESOLUTION''' | ||
| Line 26: | Line 23: | ||
[[Category: Streptomyces carzinostaticus]] | [[Category: Streptomyces carzinostaticus]] | ||
[[Category: Teplyakov, A.]] | [[Category: Teplyakov, A.]] | ||
[[Category: | [[Category: Antibacterial protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:46:34 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 02:46, 3 May 2008
CRYSTAL STRUCTURE OF APO-NEOCARZINOSTATIN AT 0.15 NM RESOLUTION
OverviewOverview
The three-dimensional structure of apo-neocarzinostatin, an antitumour antibiotic protein isolated from Streptomyces carzinostaticus, has been determined by X-ray diffraction at 0.15-nm resolution and refined to R = 17.2%. The crystal structure of neocarzinostatin is similar to that of the related proteins actinoxanthin and macromomycin. It is also in good agreement with the solution structure determined by NMR spectroscopy. The protein molecule consists of a seven-stranded antiparallel beta-sandwich and a smaller lobe formed by two beta-ribbons. A deep cleft between the two lobes is a putative chromophore binding site. Side chains of Trp39, Leu45, Phe52, Phe78 and the disulphide Cys37-Cys47 aligning the binding cleft in neocarzinostatin suggest the importance of hydrophobic interactions in stabilizing the chromophore molecule. Comparison of the atomic models of neocarzinostatin, actinoxanthin and macromomycin reveals functional residues which might determine specificity towards different chromophores.
About this StructureAbout this Structure
1NOA is a Single protein structure of sequence from Streptomyces carzinostaticus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of apo-neocarzinostatin at 0.15-nm resolution., Teplyakov A, Obmolova G, Wilson K, Kuromizu K, Eur J Biochem. 1993 Apr 15;213(2):737-41. PMID:8477746 Page seeded by OCA on Sat May 3 02:46:34 2008