1nli: Difference between revisions

Revision as of 02:40, 3 May 2008

Complex of [E160A-E189A] trichosanthin and adenine

OverviewOverview

Trichosanthin is a ribosome-inactivating protein that cleaves specifically the N-glycosidic bond of A-4324 of 28S rRNA. Trichosanthin and its variant [E160A-E189A]-trichosanthin were found to bind an adenine base with a K(d) value of approximately 0.2mM. To determine how this doubly mutated variant of trichosanthin interacts with adenine, the co-crystal structure of [E160A-E189A]-trichosanthin and adenine was resolved to 0.193nm which revealed that the active site conformation of the doubly mutated variant is isomorphous to wild-type trichosanthin. Water molecules were found at locations corresponding to the eliminated side chain of Glu-160 and Glu-189. On the other hand, the adenine base interacted with [E160A-E189A]-trichosanthin in a manner similar to that in wild-type trichosanthin. Our structural analysis illustrates that Glu-160 and Glu-189 in trichosanthin do not play an important role in maintaining the active site conformation and binding adenine, an essential step for substrate-enzyme interaction. On the other hand, removal of two glutamate residues changed a large patch of negatively charged surface to a positive charge, which may account for the destabilization of the oxocarbenium-like transition-state and the significant decrease in ribosome-inactivating activity in [E160A-E189A]-trichosanthin.

About this StructureAbout this Structure

1NLI is a Single protein structure of sequence from Trichosanthes kirilowii. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the interaction of [E160A-E189A]-trichosanthin with adenine., Shaw PC, Wong KB, Chan DS, Williams RL, Toxicon. 2003 Apr;41(5):575-81. PMID:12676436 Page seeded by OCA on Sat May 3 02:40:26 2008

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OCA

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 [[Image:1nli.jpg|left|200px]]
- {{Structure
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-|LIGAND= <scene name='pdbligand=ADE:ADENINE'>ADE</scene>
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= Trichosanthin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3677 Trichosanthes kirilowii])
+-->
-|DOMAIN=
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-}}
 '''Complex of [E160A-E189A] trichosanthin and adenine'''
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 [[Category: Single protein]]
 [[Category: Trichosanthes kirilowii]]
-[[Category: rRNA N-glycosylase]]
+[[Category: RRNA N-glycosylase]]
 [[Category: Chan, D S.B.]]
 [[Category: Shaw, P C.]]
 [[Category: Williams, R L.]]
 [[Category: Wong, K B.]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
-[[Category: ribosome-inactivating protein]]
+[[Category: Ribosome-inactivating protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:40:26 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:31:59 2008''