1nhv: Difference between revisions

Revision as of 02:32, 3 May 2008

Hepatitis C virus RNA polymerase in complex with non-nucleoside analogue inhibitor

OverviewOverview

X-ray crystal structures of two non-nucleoside analogue inhibitors bound to hepatitis C virus NS5B RNA-dependent RNA polymerase have been determined to 2.0 and 2.9 A resolution. These noncompetitive inhibitors bind to the same site on the protein, approximately 35 A from the active site. The common features of binding include a large hydrophobic region and two hydrogen bonds between both oxygen atoms of a carboxylate group on the inhibitor and two main chain amide nitrogen atoms of Ser(476) and Tyr(477) on NS5B. The inhibitor-binding site lies at the base of the thumb domain, near its interface with the C-terminal extension of NS5B. The location of this inhibitor-binding site suggests that the binding of these inhibitors interferes with a conformational change essential for the activity of the polymerase.

About this StructureAbout this Structure

1NHV is a Single protein structure of sequence from Hepatitis c virus. Full crystallographic information is available from OCA.

ReferenceReference

Non-nucleoside analogue inhibitors bind to an allosteric site on HCV NS5B polymerase. Crystal structures and mechanism of inhibition., Wang M, Ng KK, Cherney MM, Chan L, Yannopoulos CG, Bedard J, Morin N, Nguyen-Ba N, Alaoui-Ismaili MH, Bethell RC, James MN, J Biol Chem. 2003 Mar 14;278(11):9489-95. Epub 2002 Dec 30. PMID:12509436 Page seeded by OCA on Sat May 3 02:32:50 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1nhv.gif|left|200px]]
- {{Structure
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-}}
 '''Hepatitis C virus RNA polymerase in complex with non-nucleoside analogue inhibitor'''
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 [[Category: Wang, M.]]
 [[Category: Yannopoulos, C G.]]
-[[Category: enzyme inhibition]]
+[[Category: Enzyme inhibition]]
-[[Category: hepatitis c]]
+[[Category: Hepatitis c]]
-[[Category: rna polymerase]]
+[[Category: Rna polymerase]]
-[[Category: x-ray crystallography]]
+[[Category: X-ray crystallography]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:32:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:30:36 2008''