4mi7: Difference between revisions
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==Crystal Structure of Salmonella Effector Protein GtgE== | ==Crystal Structure of Salmonella Effector Protein GtgE== | ||
<StructureSection load='4mi7' size='340' side='right' caption='[[4mi7]], [[Resolution|resolution]] 1.65Å' scene=''> | <StructureSection load='4mi7' size='340' side='right'caption='[[4mi7]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4mi7]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4mi7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._SL1344 Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MI7 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mi7 OCA], [https://pdbe.org/4mi7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mi7 RCSB], [https://www.ebi.ac.uk/pdbsum/4mi7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mi7 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/H9L447_SALTY H9L447_SALTY] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]] | ||
[[Category: | [[Category: Kohler AC]] | ||
[[Category: | [[Category: Stebbins CE]] | ||
Revision as of 12:39, 28 December 2022
Crystal Structure of Salmonella Effector Protein GtgECrystal Structure of Salmonella Effector Protein GtgE
Structural highlights
FunctionPublication Abstract from PubMedGtgE is an effector protein from Salmonella Typhimurium that modulates trafficking of the Salmonella-containing vacuole. It exerts its function by cleaving the Rab-family GTPases Rab29, Rab32 and Rab38, thereby preventing the delivery of antimicrobial factors to the bacteria-containing vacuole. Here, the crystal structure of GtgE at 1.65 A resolution is presented, and structure-based mutagenesis and in vivo infection assays are used to identify its catalytic triad. A panel of cysteine protease inhibitors were examined and it was determined that N-ethylmaleimide, antipain and chymostatin inhibit GtgE activity in vitro. These findings provide the basis for the development of novel therapeutic strategies to combat Salmonella infections. Structural and enzymatic characterization of a host-specificity determinant from Salmonella.,Kohler AC, Spano S, Galan JE, Stebbins CE Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):384-91. doi:, 10.1107/S1399004713028393. Epub 2014 Jan 29. PMID:24531472[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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