1ncs: Difference between revisions

Revision as of 02:22, 3 May 2008

NMR STUDY OF SWI5 ZINC FINGER DOMAIN 1

OverviewOverview

BACKGROUND: The 2Cys-2His (C2-H2) zinc finger is a protein domain commonly used for sequence-specific DNA recognition. The zinc fingers of the yeast transcription factors SWI5 and ACE2 share strong sequence homology, which extends into a region N-terminal to the first finger, suggesting that the DNA-binding domains of these two proteins include additional structural elements. RESULTS: Structural analysis of the zinc fingers of SWI5 reveals that a 15 residue region N-terminal to the finger motifs forms part of the structure of the first finger domain, adding a beta strand and a helix not previously observed in other zinc finger structures. Sequence analysis suggests that other zinc finger proteins may also have this structure. Biochemical studies show that this additional structure increases DNA-binding affinity. CONCLUSIONS: The structural analysis presented reveals a novel zinc finger structure in which additional structural elements have been added to the C2-H2 zinc finger fold. This additional structure may enhance stability and has implications for DNA recognition by extending the potential DNA-binding surface of a single zinc finger domain.

About this StructureAbout this Structure

1NCS is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

The solution structure of the first zinc finger domain of SWI5: a novel structural extension to a common fold., Dutnall RN, Neuhaus D, Rhodes D, Structure. 1996 May 15;4(5):599-611. PMID:8736557 Page seeded by OCA on Sat May 3 02:22:43 2008

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OCA

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 [[Image:1ncs.jpg|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_1ncs", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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-|GENE= T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+-->
-|DOMAIN=
+{{STRUCTURE_1ncs|  PDB=1ncs  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ncs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ncs OCA], [http://www.ebi.ac.uk/pdbsum/1ncs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ncs RCSB]</span>
-}}
 '''NMR STUDY OF SWI5 ZINC FINGER DOMAIN 1'''
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 [[Category: Neuhaus, D.]]
 [[Category: Rhodes, D.]]
-[[Category: dna binding motif]]
+[[Category: Dna binding motif]]
-[[Category: transcription regulation]]
+[[Category: Transcription regulation]]
-[[Category: zinc-finger]]
+[[Category: Zinc-finger]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:22:43 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:28:38 2008''