1n87: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1n87.gif|left|200px]] | [[Image:1n87.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1n87", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_1n87| PDB=1n87 | SCENE= }} | |||
| | |||
}} | |||
'''Solution structure of the U-box of Prp19''' | '''Solution structure of the U-box of Prp19''' | ||
| Line 28: | Line 25: | ||
[[Category: Kooi, C W.Vander.]] | [[Category: Kooi, C W.Vander.]] | ||
[[Category: Ohi, M D.]] | [[Category: Ohi, M D.]] | ||
[[Category: | [[Category: E3 ligase]] | ||
[[Category: | [[Category: U-box]] | ||
[[Category: | [[Category: Ubiquitin ligase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:13:01 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 02:13, 3 May 2008
Solution structure of the U-box of Prp19
OverviewOverview
The structure of the U-box in the essential Saccharomyces cerevisiae pre-mRNA splicing factor Prp19p has been determined by NMR. The conserved zinc-binding sites supporting the cross-brace arrangement in RING-finger domains are replaced by hydrogen-bonding networks in the U-box. These hydrogen-bonding networks are necessary for the structural stabilization and activity of the U-box. A conservative Val-->Ile point mutation in the Prp19p U-box domain leads to pre-mRNA splicing defects in vivo. NMR analysis of this mutant shows that the substitution disrupts structural integrity of the U-box domain. Furthermore, comparison of the Prp19p U-box domain with known RING-E2 complex structures demonstrates that both U-box and RING-fingers contain a conserved interaction surface. Mutagenesis of residues at this interface, while not perturbing the structure of the U-box, abrogates Prp19p function in vivo. These comparative structural and functional analyses imply that the U-box and its associated ubiquitin ligase activity are critical for Prp19p function in vivo.
About this StructureAbout this Structure
1N87 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Structural insights into the U-box, a domain associated with multi-ubiquitination., Ohi MD, Vander Kooi CW, Rosenberg JA, Chazin WJ, Gould KL, Nat Struct Biol. 2003 Apr;10(4):250-5. PMID:12627222 Page seeded by OCA on Sat May 3 02:13:01 2008