1n6y: Difference between revisions

Revision as of 02:10, 3 May 2008

RIP-phasing on Bovine Trypsin

OverviewOverview

The use of third generation synchrotron sources has led to renewed concern about the effect of ionizing radiation on crystalline biological samples. In general, the problem is seen as one to be avoided. However, in this paper, it is shown that, far from being a hindrance to successful structure determination, radiation damage provides an opportunity for phasing macromolecular structures. This is successfully demonstrated for both a protein and an oligonucleotide, by way of which complete models were built automatically. The possibility that, through the exploitation of radiation damage, the phase problem could become less of a barrier to macromolecular crystal structure determination is discussed.

About this StructureAbout this Structure

1N6Y is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

Specific radiation damage can be used to solve macromolecular crystal structures., Ravelli RB, Leiros HK, Pan B, Caffrey M, McSweeney S, Structure. 2003 Feb;11(2):217-24. PMID:12575941 Page seeded by OCA on Sat May 3 02:10:22 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1n6y.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1n6y |SIZE=350|CAPTION= <scene name='initialview01'>1n6y</scene>, resolution 1.40&Aring;
+The line below this paragraph, containing "STRUCTURE_1n6y", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ABN:BENZYLAMINE'>ABN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1n6y|  PDB=1n6y  |  SCENE=  }}
-|RELATEDENTRY=[[1n6x|1N6X]], [[1n7a|1N7A]], [[1n7b|1N7B]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n6y OCA], [http://www.ebi.ac.uk/pdbsum/1n6y PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n6y RCSB]</span>
-}}
 '''RIP-phasing on Bovine Trypsin'''
@@ Line 31: / Line 28: @@
 [[Category: Pan, B.]]
 [[Category: Ravelli, R B.G.]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:10:22 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:26:15 2008''